Detailed information of TA system TA175893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4468675..4469369
• Replicon: chromosome
• Accession: NZ_CP062731
• Organism: Escherichia coli O157:H7 strain Z1768

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ48_RS22540
• Protein ID: WP_001263495.1
• Coordinates: 4468675..4469073 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ48_RS22545
• Protein ID: WP_000554757.1
• Coordinates: 4469076..4469369 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4464262..4464342	-	81	NuclAT_11	-	-
-	4464262..4464342	-	81	NuclAT_11	-	-
-	4464262..4464342	-	81	NuclAT_11	-	-
-	4464262..4464342	-	81	NuclAT_11	-	-
ILQ48_RS22515	4464938..4465396	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ48_RS22520	4465657..4467114	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ48_RS22525	4467171..4467692	-	522	Protein_4409	peptide chain release factor H	-
ILQ48_RS22530	4467691..4467894	-	204	Protein_4410	RNA ligase RtcB family protein	-
ILQ48_RS22535	4468213..4468665	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ48_RS22540	4468675..4469073	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ48_RS22545	4469076..4469369	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ48_RS22550	4469421..4470476	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ48_RS22555	4470547..4471332	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ48_RS22560	4471304..4473016	+	1713	Protein_4416	flagellar biosynthesis protein FlhA	-
ILQ48_RS22565	4473161..4473658	-	498	Protein_4417	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4430520..4474607	44087

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175893 WP_001263495.1 NZ_CP062731:c4469073-4468675 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175893 NZ_CP082238:c175223-174927 [Acidithiobacillus ferrooxidans]
ATGCTTGAGCTCATAGAAACCAACGTGTTCAGGGAATGGCTCGAAGGTCTGCGCGACCACCAAGCCCGCGCAAGGATTCA
GGTGCGGATGCGCCGATTGAGCCTTGGGAACCCTGGAGACGTGAAGCCTGTAGGTGAAGGCATCTCGGAAATGCGGATTG
ACTACGGTCCTGGCTATCGCGTCTATTTCATGAGGCGCGGACCAGTTGTGGTGGCGCTTCTGTGCGGCGGAGACAAGGGG
AGCCAGGAACGGGACATTGCTTTGGCAAAAGCCATTGCAGCGCAATGGAAGGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175893 WP_000554757.1 NZ_CP062731:c4469369-4469076 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175893 NZ_CP082238:c174921-174604 [Acidithiobacillus ferrooxidans]
ATGGAAGTGCAATTCAGACGCTACGACACCGTGAATTACCTCAAGACTGAGGACGATATTGCGCACTATCTCGATGCTGC
GATGGAGGATGGCGACCCCGCGTTGATTCTTGCAGCGCTGGGAGACGTTGCCCGTGCCCGCAACCTCAGCCAGCTCGCCC
GCGACGCAGGCATGAGCCGCCAAGGATTGGACAAGGCCCTTTCCGGAACAGGGAACCCAAGCCTGGTAACGGTGCTGAAA
ATAACCCAGGCACTCGGCCTGCGGCTTTCCTTCCAACCAGCACACACAAGCAGTCACCCATCGGCTACAGGGGTTTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6