Detailed information of TA system TA175858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4472344..4472883
• Replicon: chromosome
• Accession: NZ_CP062729
• Organism: Escherichia coli O157:H7 strain Z1811

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: ILQ50_RS22570
• Protein ID: WP_001303998.1
• Coordinates: 4472623..4472883 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: ILQ50_RS22565
• Protein ID: WP_000729705.1
• Coordinates: 4472344..4472604 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ50_RS22545	4468098..4468883	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ50_RS22550	4468855..4470567	+	1713	Protein_4414	flagellar biosynthesis protein FlhA	-
ILQ50_RS22555	4470712..4471209	-	498	Protein_4415	REP-associated tyrosine transposase RayT	-
ILQ50_RS22560	4471385..4472158	-	774	WP_000543891.1	C40 family peptidase	-
ILQ50_RS22565	4472344..4472604	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
ILQ50_RS22570	4472623..4472883	+	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
ILQ50_RS22575	4473039..4473779	+	741	WP_001225679.1	murein L,D-transpeptidase	-
ILQ50_RS22580	4473750..4474517	-	768	WP_001301698.1	class II glutamine amidotransferase	-
ILQ50_RS22585	4474622..4475200	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T175858 WP_001303998.1 NZ_CP062729:4472623-4472883 [Escherichia coli O157:H7]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T175858 NZ_CP082217:c4217786-4217634 [Xanthomonas cucurbitae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGCGCCGGCAACAAGGTCGAGAAGACGGCCGAAAAATGCAGCGACGGTAAGTGCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT175858 WP_000729705.1 NZ_CP062729:4472344-4472604 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT175858 NZ_CP082217:c4217556-4217422 [Xanthomonas cucurbitae]
ATGAAGCGTGCAATCGTGCTGCTGGTGCTGTCAGTGTTGTCGGTCGGGATGCTGTCAGGTTGCAACACCGTCGCAGGTGC
CGGCAAAGACGTGCAGGGCGCCGGCGAGAAGGTCGAAGACGCCGCACGCAAGTGA