Detailed information of TA system TA175857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4466226..4466920
• Replicon: chromosome
• Accession: NZ_CP062729
• Organism: Escherichia coli O157:H7 strain Z1811

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ50_RS22530
• Protein ID: WP_001263495.1
• Coordinates: 4466226..4466624 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ50_RS22535
• Protein ID: WP_000554757.1
• Coordinates: 4466627..4466920 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4461813..4461893	-	81	NuclAT_11	-	-
-	4461813..4461893	-	81	NuclAT_11	-	-
-	4461813..4461893	-	81	NuclAT_11	-	-
-	4461813..4461893	-	81	NuclAT_11	-	-
ILQ50_RS22505	4462489..4462947	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ50_RS22510	4463208..4464665	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ50_RS22515	4464722..4465243	-	522	Protein_4407	peptide chain release factor H	-
ILQ50_RS22520	4465242..4465445	-	204	Protein_4408	RNA ligase RtcB family protein	-
ILQ50_RS22525	4465764..4466216	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ50_RS22530	4466226..4466624	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ50_RS22535	4466627..4466920	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ50_RS22540	4466972..4468027	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ50_RS22545	4468098..4468883	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ50_RS22550	4468855..4470567	+	1713	Protein_4414	flagellar biosynthesis protein FlhA	-
ILQ50_RS22555	4470712..4471209	-	498	Protein_4415	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175857 WP_001263495.1 NZ_CP062729:c4466624-4466226 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175857 NZ_CP082217:c4217556-4217422 [Xanthomonas cucurbitae]
ATGAAGCGTGCAATCGTGCTGCTGGTGCTGTCAGTGTTGTCGGTCGGGATGCTGTCAGGTTGCAACACCGTCGCAGGTGC
CGGCAAAGACGTGCAGGGCGCCGGCGAGAAGGTCGAAGACGCCGCACGCAAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175857 WP_000554757.1 NZ_CP062729:c4466920-4466627 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175857 NZ_CP082217:c4217786-4217634 [Xanthomonas cucurbitae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGCGCCGGCAACAAGGTCGAGAAGACGGCCGAAAAATGCAGCGACGGTAAGTGCTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6