Detailed information of TA system TA175848

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3009056..3009281
• Replicon: chromosome
• Accession: NZ_CP062729
• Organism: Escherichia coli O157:H7 strain Z1811

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ50_RS15160
• Protein ID: WP_000813258.1
• Coordinates: 3009056..3009211 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3009223..3009281 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ50_RS15110	3004059..3004490	-	432	WP_001302123.1	tellurite resistance protein	-
ILQ50_RS15125	3004941..3005654	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ50_RS15130	3005790..3005987	-	198	WP_000917763.1	hypothetical protein	-
ILQ50_RS15135	3006212..3006766	-	555	WP_000640035.1	DUF1133 family protein	-
ILQ50_RS15140	3006829..3007134	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ50_RS15145	3007147..3008196	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ50_RS15150	3008198..3008470	-	273	WP_000191872.1	hypothetical protein	-
ILQ50_RS15155	3008592..3008936	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ50_RS15160	3009056..3009211	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	3009223..3009281	+	59	-	-	Antitoxin
ILQ50_RS15165	3009502..3010059	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ILQ50_RS15170	3010061..3010279	-	219	WP_000683609.1	DUF4014 family protein	-
ILQ50_RS15175	3010407..3010718	-	312	WP_001289673.1	hypothetical protein	-
ILQ50_RS15180	3010711..3010938	-	228	WP_000699809.1	hypothetical protein	-
ILQ50_RS15185	3010935..3011216	-	282	WP_000603384.1	DNA-binding protein	-
ILQ50_RS15190	3011249..3011965	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ50_RS15195	3011999..3012460	-	462	WP_000139447.1	replication protein	-
ILQ50_RS15200	3012453..3013496	-	1044	WP_001262402.1	hypothetical protein	-
ILQ50_RS15205	3013565..3013990	-	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ50_RS15210	3013974..3014216	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC / nleG7'	2926140..3069044	142904

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175848 WP_000813258.1 NZ_CP062729:c3009211-3009056 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175848 NZ_CP082215:3616722-3617372 [Xanthomonas cucurbitae]
ATGTCTAGGCGCTACCTCAACCTCAATCCAGACAAAGCCCTGATCTGGCGCATCGTCCACCGCGATAATCTGCCTTGGAT
TCTGGACAACGGCCTGCATTGCGGTAACGGCAGCGCGCGTAGTCCGGGCTGGGTAAGCATCGGCAATCCTGAATTGATCG
ACAAGCGCGCCTGTCATCCTGTGCCGCTTACACCCGGCGGCTTCCTGAACGATTACGTGCCCTTCTACTTCACTCCATTT
TCGCCAATGCTTCGCAACATCCAGACCGGCTGGGGAGGCATCCAACAACGCGCTAACGATGAAATTGTGATCCTTGTGTC
CAGCTTGCATCATGTCAAGGCACGTGGGTGGCCCTTCCTGTTCACCGATAGTCATGCCTACTATCAGTTGACAACGTTCC
ATTCCGATTTGACCAATCTGGACACGATCGACTGGTCGCTGATCCAGGCGCGCGATTTTCGCCGCGATCAGGAGGATCCG
GCCAAGTTCGAACGATATCAGGCCGAAGCGCTGGTCCACCGACACCTGCCCGTCGACGGCTTGCTCGGCATCGTGTGTTA
TACGGACGACATGAAGCGTTCCATCGAACGTCAGCTTGAAGCCCGAAACCTTGCACTTTCCGTGCATGCCCGCACCGAGT
GGTACTTCTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175848 NZ_CP062729:3009223-3009281 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin