Detailed information of TA system TA175821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4468249..4468943
• Replicon: chromosome
• Accession: NZ_CP062727
• Organism: Escherichia coli O157:H7 strain Z1812

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ51_RS22510
• Protein ID: WP_001263495.1
• Coordinates: 4468249..4468647 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ51_RS22515
• Protein ID: WP_000554757.1
• Coordinates: 4468650..4468943 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4463836..4463916	-	81	NuclAT_11	-	-
-	4463836..4463916	-	81	NuclAT_11	-	-
-	4463836..4463916	-	81	NuclAT_11	-	-
-	4463836..4463916	-	81	NuclAT_11	-	-
ILQ51_RS22485	4464512..4464970	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ51_RS22490	4465231..4466688	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ51_RS22495	4466745..4467266	-	522	Protein_4403	peptide chain release factor H	-
ILQ51_RS22500	4467265..4467468	-	204	Protein_4404	RNA ligase RtcB family protein	-
ILQ51_RS22505	4467787..4468239	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ51_RS22510	4468249..4468647	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ51_RS22515	4468650..4468943	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ51_RS22520	4468995..4470050	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ51_RS22525	4470121..4470906	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ51_RS22530	4470878..4472590	+	1713	Protein_4410	flagellar biosynthesis protein FlhA	-
ILQ51_RS22535	4472735..4473232	-	498	Protein_4411	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4430117..4474181	44064

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175821 WP_001263495.1 NZ_CP062727:c4468647-4468249 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175821 NZ_CP082212:364418-364786 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175821 WP_000554757.1 NZ_CP062727:c4468943-4468650 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175821 NZ_CP082212:364200-364421 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6