Detailed information of TA system TA175816

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3581666..3581891
• Replicon: chromosome
• Accession: NZ_CP062727
• Organism: Escherichia coli O157:H7 strain Z1812

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ51_RS18395
• Protein ID: WP_000813263.1
• Coordinates: 3581666..3581821 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3581833..3581891 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ51_RS18360	3577120..3577833	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ51_RS18365	3577971..3578167	-	197	Protein_3594	TrmB family transcriptional regulator	-
ILQ51_RS18370	3578454..3579272	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ51_RS18375	3579424..3579795	-	372	WP_000090264.1	antitermination protein	-
ILQ51_RS18380	3579785..3580156	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ51_RS18385	3580169..3581218	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ51_RS18390	3581220..3581498	-	279	WP_001341388.1	hypothetical protein	-
ILQ51_RS18395	3581666..3581821	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3581833..3581891	+	59	-	-	Antitoxin
ILQ51_RS18400	3582426..3583199	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ51_RS18405	3583551..3583964	-	414	WP_001151233.1	DUF977 family protein	-
ILQ51_RS18410	3583980..3584750	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ51_RS18415	3584772..3585518	-	747	WP_000788751.1	ATP-binding protein	-
ILQ51_RS18420	3585525..3586616	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T175816 WP_000813263.1 NZ_CP062727:c3581821-3581666 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T175816 NZ_CP082211:c4670072-4669539 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175816 NZ_CP062727:3581833-3581891 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin