Detailed information of TA system TA175785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4546252..4546946
• Replicon: chromosome
• Accession: NZ_CP062725
• Organism: Escherichia coli O157:H7 strain Z1813

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ52_RS23080
• Protein ID: WP_001263495.1
• Coordinates: 4546252..4546650 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ52_RS23085
• Protein ID: WP_000554757.1
• Coordinates: 4546653..4546946 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4541839..4541919	-	81	NuclAT_12	-	-
-	4541839..4541919	-	81	NuclAT_12	-	-
-	4541839..4541919	-	81	NuclAT_12	-	-
-	4541839..4541919	-	81	NuclAT_12	-	-
ILQ52_RS23055	4542515..4542973	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ52_RS23060	4543234..4544691	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ52_RS23065	4544748..4545269	-	522	Protein_4516	peptide chain release factor H	-
ILQ52_RS23070	4545268..4545471	-	204	Protein_4517	RNA ligase RtcB family protein	-
ILQ52_RS23075	4545790..4546242	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ52_RS23080	4546252..4546650	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ52_RS23085	4546653..4546946	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ52_RS23090	4546998..4548053	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ52_RS23095	4548124..4548909	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ52_RS23100	4548881..4550593	+	1713	Protein_4523	flagellar biosynthesis protein FlhA	-
ILQ52_RS23105	4550738..4551235	-	498	Protein_4524	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175785 WP_001263495.1 NZ_CP062725:c4546650-4546252 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175785 NZ_CP082202:c2097178-2096849 [Streptococcus suis]
ATGGAATTTGAGTCATATTCAGTGATGCTTGCTCCTGCAGTGGAGAAGGAATTGGCAGCTATTTATGCCTATTTCTCGGA
ACAGTTCTCAGAAGAAATTGCCAAAAGACGAATTGGGATGATTGTTGAAGCTTTGGAGAGTTTGCAGATTTTTCCTGAAC
GTGGTTTTAACGCTGATAACCGTTTTGGTAAGCAGGTAGACCCGCCACATCTAACCCGAGGTTACGTTATTGGAAAAGAT
TACATCGTCCTCTATCGCGTAGTGAAAAATGAAGTTCGAGTTGGACACCTTTTTGCGACCAAGAGTGATTATGTAAAGTT
ATTGAAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175785 WP_000554757.1 NZ_CP062725:c4546946-4546653 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175785 NZ_CP082202:c2097455-2097168 [Streptococcus suis]
TTGCTAACTATAAAGAAAGATGCCCAAGTAAATTTTAAAACCGACTCTAGACTTTTAGAGAAAGCCAAGGAAGTATTTGC
TATGAACCAGCTGGATTTGACTGCTGGTTTTAACCTCTTCTTGCAAAATATTGCTGTAAAAAATGAGTTGCCCATTCTGA
CTGAAGAAGAGTTGGAAAGAGAAGAGTTGTTTTTACAGTTACAGAAGGAAATTCAGGAAAATCAGCAGGCGATTGAGGCA
GGTCATGGGATCAGCCTAGAAAGTGTCAAAGAGAAGTATGGAATTTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6