Detailed information of TA system TA175750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4480292..4480831
• Replicon: chromosome
• Accession: NZ_CP062723
• Organism: Escherichia coli O157:H7 strain Z1814

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: ILQ53_RS22620
• Protein ID: WP_001303998.1
• Coordinates: 4480571..4480831 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: ILQ53_RS22615
• Protein ID: WP_000729705.1
• Coordinates: 4480292..4480552 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ53_RS22595	4476046..4476831	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ53_RS22600	4476803..4478515	+	1713	Protein_4425	flagellar biosynthesis protein FlhA	-
ILQ53_RS22605	4478660..4479157	-	498	Protein_4426	REP-associated tyrosine transposase RayT	-
ILQ53_RS22610	4479333..4480106	-	774	WP_000543891.1	C40 family peptidase	-
ILQ53_RS22615	4480292..4480552	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
ILQ53_RS22620	4480571..4480831	+	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
ILQ53_RS22625	4480987..4481727	+	741	WP_001225679.1	murein L,D-transpeptidase	-
ILQ53_RS22630	4481698..4482465	-	768	WP_001301698.1	class II glutamine amidotransferase	-
ILQ53_RS22635	4482570..4483148	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T175750 WP_001303998.1 NZ_CP062723:4480571-4480831 [Escherichia coli O157:H7]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T175750 NZ_CP082178:529756-529890 [Xanthomonas fragariae]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTTTCGGTGTTGTCGGTCGGTATGCTGTCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCTGGCGAGAAAGTGGAAGACGCTGCGCGCAAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT175750 WP_000729705.1 NZ_CP062723:4480292-4480552 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT175750 NZ_CP082178:529524-529676 [Xanthomonas fragariae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGTCGGCGTGATGACTGGCTGTAACACCGTTGCTGGCGC
CGGCAAGGACATCCAACGCGCGGGCAGCAAGGTCGAAAAGAAGGCCGATAACTGCAGCGACGGTAAATGCTGA