Detailed information of TA system TA175749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4474174..4474868
• Replicon: chromosome
• Accession: NZ_CP062723
• Organism: Escherichia coli O157:H7 strain Z1814

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ53_RS22580
• Protein ID: WP_001263495.1
• Coordinates: 4474174..4474572 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ53_RS22585
• Protein ID: WP_000554757.1
• Coordinates: 4474575..4474868 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4469761..4469841	-	81	NuclAT_11	-	-
-	4469761..4469841	-	81	NuclAT_11	-	-
-	4469761..4469841	-	81	NuclAT_11	-	-
-	4469761..4469841	-	81	NuclAT_11	-	-
ILQ53_RS22555	4470437..4470895	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ53_RS22560	4471156..4472613	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ53_RS22565	4472670..4473191	-	522	Protein_4418	peptide chain release factor H	-
ILQ53_RS22570	4473190..4473393	-	204	Protein_4419	RNA ligase RtcB family protein	-
ILQ53_RS22575	4473712..4474164	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ53_RS22580	4474174..4474572	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ53_RS22585	4474575..4474868	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ53_RS22590	4474920..4475975	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ53_RS22595	4476046..4476831	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ53_RS22600	4476803..4478515	+	1713	Protein_4425	flagellar biosynthesis protein FlhA	-
ILQ53_RS22605	4478660..4479157	-	498	Protein_4426	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175749 WP_001263495.1 NZ_CP062723:c4474572-4474174 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175749 NZ_CP082178:529524-529676 [Xanthomonas fragariae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGTCGGCGTGATGACTGGCTGTAACACCGTTGCTGGCGC
CGGCAAGGACATCCAACGCGCGGGCAGCAAGGTCGAAAAGAAGGCCGATAACTGCAGCGACGGTAAATGCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175749 WP_000554757.1 NZ_CP062723:c4474868-4474575 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175749 NZ_CP082178:529756-529890 [Xanthomonas fragariae]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTTTCGGTGTTGTCGGTCGGTATGCTGTCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCTGGCGAGAAAGTGGAAGACGCTGCGCGCAAGTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6