Detailed information of TA system TA175744

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3577675..3577900
• Replicon: chromosome
• Accession: NZ_CP062723
• Organism: Escherichia coli O157:H7 strain Z1814

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ53_RS18380
• Protein ID: WP_000813263.1
• Coordinates: 3577675..3577830 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3577842..3577900 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ53_RS18345	3573129..3573842	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ53_RS18350	3573980..3574176	-	197	Protein_3592	TrmB family transcriptional regulator	-
ILQ53_RS18355	3574463..3575281	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ53_RS18360	3575433..3575804	-	372	WP_000090264.1	antitermination protein	-
ILQ53_RS18365	3575794..3576165	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ53_RS18370	3576178..3577227	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ53_RS18375	3577229..3577507	-	279	WP_001341388.1	hypothetical protein	-
ILQ53_RS18380	3577675..3577830	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3577842..3577900	+	59	-	-	Antitoxin
ILQ53_RS18385	3578435..3579208	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ53_RS18390	3579560..3579973	-	414	WP_001151233.1	DUF977 family protein	-
ILQ53_RS18395	3579989..3580759	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ53_RS18400	3580781..3581527	-	747	WP_000788751.1	ATP-binding protein	-
ILQ53_RS18405	3581534..3582625	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T175744 WP_000813263.1 NZ_CP062723:c3577830-3577675 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T175744 NZ_CP082164:7873079-7873504 [Bradyrhizobium sp. 186]
GTGAACCTACTGCTCGACACCAACGTGTTGTCGGAAGTTCAGCGGCCGGCGCCCGATTCGAAAGTTCTGGCCTGGTTGGA
CACCGTCGACGAGGACCGCGCGTTCATCAGCGTCGCCTCGATCGCCGAGCTTCGGCGCGGCATCGCGCTGATGGAAGACG
GCCGCCGGCGCACGGCGTTGGCCGCCTGGCTCGCCCACAATCTGCCAACACGGTTTGCCGGCCGCATCCTGCCGATCGAT
CACGCGGTGGCCGAACGCTGGGGCGACCTGATGGCGCAGAGCCGCAGAAGCGGCGTCGCGCTGTCCGTCATGGACGGCTT
CTTTGCCGCGACCGCGCTCGCAAACAACCTCACGCTCGTCACGCGCAATGTGAAGGATTTAGCGGCATTCGGCGTCCCGC
TGCTCAACCCGTGGGACGACGCATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175744 NZ_CP062723:3577842-3577900 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin