Detailed information of TA system TA175739

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2648150..2648375
• Replicon: chromosome
• Accession: NZ_CP062723
• Organism: Escherichia coli O157:H7 strain Z1814

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ53_RS13440
• Protein ID: WP_000813258.1
• Coordinates: 2648220..2648375 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2648150..2648208 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ53_RS13390	2643216..2643457	+	242	Protein_2615	hypothetical protein	-
ILQ53_RS13395	2643441..2643866	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ53_RS13400	2643935..2644978	+	1044	WP_001262402.1	hypothetical protein	-
ILQ53_RS13405	2644971..2645432	+	462	WP_000139447.1	replication protein	-
ILQ53_RS13410	2645466..2646182	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ53_RS13415	2646215..2646496	+	282	WP_000603384.1	DNA-binding protein	-
ILQ53_RS13420	2646493..2646720	+	228	WP_000699809.1	hypothetical protein	-
ILQ53_RS13425	2646713..2647024	+	312	WP_001289673.1	hypothetical protein	-
ILQ53_RS13430	2647152..2647370	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ53_RS13435	2647372..2647929	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2648150..2648208	-	59	-	-	Antitoxin
ILQ53_RS13440	2648220..2648375	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ53_RS13445	2648495..2648839	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ53_RS13450	2648961..2649233	+	273	WP_000191872.1	hypothetical protein	-
ILQ53_RS13455	2649235..2650284	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ53_RS13460	2650297..2650602	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ53_RS13465	2650665..2651219	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ53_RS13470	2651444..2651641	+	198	WP_000917763.1	hypothetical protein	-
ILQ53_RS13475	2651776..2652489	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ53_RS13490	2652940..2653371	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7' / nleC	2578757..2739735	160978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175739 WP_000813258.1 NZ_CP062723:2648220-2648375 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175739 NZ_CP082164:c5429599-5429204 [Bradyrhizobium sp. 186]
ATGGTCTTGGACACGTCGGCAATCATAGCGGCGATAACCAATGAGCCCGATGGTCCACGCTATCGCGCCGCAATGCGCGA
TGCCGACAATCTGCTGATCTCAGCCGTAGCAGTACTTGAAACCAGGATCGTTCTCTTCGCGCGGCTTGGACCACAAGCCG
TTGAGCTTTTCGACGAATTGCTGCGGAGCACGACACTGGTCGTGGTGCCATTTGACGACGAAATGTCGAAAGGCGCGTTC
GAGGCGTTTCGGCAGTACGGCAAGGGTAGCGGCCATCCCGCGCAGTTGAACATTGTTGATTGCGCCGTCTATGCGTTGGC
CAAGTCGCGTTCGCAGCCGCTCCTCTTCAAGGGCGGCGACTTCGGCAGGACGGATATCGCGATCGCGCTGATCTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175739 NZ_CP062723:c2648208-2648150 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin