Detailed information of TA system TA175727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 708397..709196
• Replicon: chromosome
• Accession: NZ_CP062723
• Organism: Escherichia coli O157:H7 strain Z1814

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: ILQ53_RS03455
• Protein ID: WP_000347273.1
• Coordinates: 708397..708861 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: ILQ53_RS03460
• Protein ID: WP_001302819.1
• Coordinates: 708861..709196 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ53_RS03425	703398..703832	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
ILQ53_RS03430	703850..704728	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ILQ53_RS03435	704718..705497	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ILQ53_RS03440	705508..705981	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ILQ53_RS03445	706004..707284	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ILQ53_RS03450	707533..708342	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
ILQ53_RS03455	708397..708861	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ILQ53_RS03460	708861..709196	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ILQ53_RS03465	709345..710916	-	1572	WP_001273776.1	galactarate dehydratase	-
ILQ53_RS03470	711291..712625	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
ILQ53_RS03475	712641..713411	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T175727 WP_000347273.1 NZ_CP062723:c708861-708397 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T175727 NZ_CP082154:c3036361-3036143 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT175727 WP_001302819.1 NZ_CP062723:c709196-708861 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT175727 NZ_CP082154:c3036762-3036388 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: V0SSH7