Detailed information of TA system TA175713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4514457..4515151
• Replicon: chromosome
• Accession: NZ_CP062721
• Organism: Escherichia coli O157:H7 strain Z1815

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ54_RS22865
• Protein ID: WP_001263495.1
• Coordinates: 4514457..4514855 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ54_RS22870
• Protein ID: WP_000554757.1
• Coordinates: 4514858..4515151 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4510044..4510124	-	81	NuclAT_11	-	-
-	4510044..4510124	-	81	NuclAT_11	-	-
-	4510044..4510124	-	81	NuclAT_11	-	-
-	4510044..4510124	-	81	NuclAT_11	-	-
ILQ54_RS22840	4510720..4511178	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ54_RS22845	4511439..4512896	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ54_RS22850	4512953..4513474	-	522	Protein_4473	peptide chain release factor H	-
ILQ54_RS22855	4513473..4513676	-	204	Protein_4474	RNA ligase RtcB family protein	-
ILQ54_RS22860	4513995..4514447	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ54_RS22865	4514457..4514855	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ54_RS22870	4514858..4515151	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ54_RS22875	4515203..4516258	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ54_RS22880	4516329..4517114	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ54_RS22885	4517086..4518798	+	1713	Protein_4480	flagellar biosynthesis protein FlhA	-
ILQ54_RS22890	4518943..4519440	-	498	Protein_4481	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175713 WP_001263495.1 NZ_CP062721:c4514855-4514457 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175713 NZ_CP082147:c3976909-3976499 [Enterobacter cloacae complex sp.]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCGTTAATGCTCCACGGCCTGGTTGCGGC
CATCGTGTTACTCATGCCATGGCCCCTGAGCTATACACCGCTGTGGCTGCTATTACTTTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCTCGTCAGGGTGAGATCAAGTTACTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGATATTCTGGGTATGCCATGGATGCTCAACTCCGGCATGATGTTACGGTTACGCAATGTGGACGGCGGGCGTTG
CCAGCACCTGTGGCTGGCGGCTGACAGTATGGATGCTTCAGAATGGCGAGATCTGCGCCGGATGTTGTTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175713 WP_000554757.1 NZ_CP062721:c4515151-4514858 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175713 NZ_CP082147:c3977156-3976890 [Enterobacter cloacae complex sp.]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCATGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGGCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6