Detailed information of TA system TA175708

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3627856..3628081
• Replicon: chromosome
• Accession: NZ_CP062721
• Organism: Escherichia coli O157:H7 strain Z1815

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ54_RS18750
• Protein ID: WP_000813263.1
• Coordinates: 3627856..3628011 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3628023..3628081 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ54_RS18715	3623310..3624023	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ54_RS18720	3624161..3624357	-	197	Protein_3664	TrmB family transcriptional regulator	-
ILQ54_RS18725	3624644..3625462	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ54_RS18730	3625614..3625985	-	372	WP_000090264.1	antitermination protein	-
ILQ54_RS18735	3625975..3626346	-	372	WP_032283490.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ54_RS18740	3626359..3627408	-	1050	WP_206338758.1	DUF968 domain-containing protein	-
ILQ54_RS18745	3627410..3627688	-	279	WP_001341388.1	hypothetical protein	-
ILQ54_RS18750	3627856..3628011	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3628023..3628081	+	59	-	-	Antitoxin
ILQ54_RS18755	3628616..3629389	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ54_RS18760	3629741..3630154	-	414	WP_001151233.1	DUF977 family protein	-
ILQ54_RS18765	3630170..3630940	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ54_RS18770	3630962..3631708	-	747	WP_000788751.1	ATP-binding protein	-
ILQ54_RS18775	3631715..3632806	-	1092	WP_206338777.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T175708 WP_000813263.1 NZ_CP062721:c3628011-3627856 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T175708 NZ_CP082147:c974483-974142 [Enterobacter cloacae complex sp.]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTAGCACCCGTGGCTGTCTGGCAAATGCTACTGAC
CCGCCTGCTGAAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGAATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175708 NZ_CP062721:3628023-3628081 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin