Detailed information of TA system TA175705

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3227698..3227923
• Replicon: chromosome
• Accession: NZ_CP062721
• Organism: Escherichia coli O157:H7 strain Z1815

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ54_RS16445
• Protein ID: WP_000813258.1
• Coordinates: 3227698..3227853 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3227865..3227923 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ54_RS16395	3222701..3223132	-	432	WP_001302123.1	tellurite resistance protein	-
ILQ54_RS16410	3223583..3224296	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ54_RS16415	3224432..3224629	-	198	WP_000917763.1	hypothetical protein	-
ILQ54_RS16420	3224854..3225408	-	555	WP_000640035.1	DUF1133 family protein	-
ILQ54_RS16425	3225471..3225776	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ54_RS16430	3225789..3226838	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ54_RS16435	3226840..3227112	-	273	WP_000191872.1	hypothetical protein	-
ILQ54_RS16440	3227234..3227578	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ54_RS16445	3227698..3227853	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	3227865..3227923	+	59	-	-	Antitoxin
ILQ54_RS16450	3228144..3228701	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ILQ54_RS16455	3228703..3228921	-	219	WP_000683609.1	DUF4014 family protein	-
ILQ54_RS16460	3229049..3229360	-	312	WP_001289673.1	hypothetical protein	-
ILQ54_RS16465	3229353..3229580	-	228	WP_000699809.1	hypothetical protein	-
ILQ54_RS16470	3229577..3229858	-	282	WP_000603384.1	DNA-binding protein	-
ILQ54_RS16475	3229891..3230607	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ54_RS16480	3230641..3231102	-	462	WP_000139447.1	replication protein	-
ILQ54_RS16485	3231095..3232138	-	1044	WP_001262402.1	hypothetical protein	-
ILQ54_RS16490	3232207..3232632	-	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ54_RS16495	3232616..3232858	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	3192930..3290239	97309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175705 WP_000813258.1 NZ_CP062721:c3227853-3227698 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175705 NZ_CP082147:c62089-61739 [Enterobacter cloacae complex sp.]
ATGTGGACGGTACTGTTCGGAAAGGTGTTTGAGCTATGGTTGCTTGAACAGGAAGAAGGGTTACAGGAAAAGGTGCTGGC
AGACATGCTAAATCTACAAAACTTTGGACCTCGTCTTCCTCGTCCCTATGCCGATACTGTAAAAGGCTCGCAATATAACA
ATATGAAAGAGTTACGTATTCAATATGCTGGCCGTCCGATTCGTGCTTTATTCGCATTTGACCCCGAACGTCAGGCGATT
GTGCTTTGCGCAGGCAACAAGAGTAACGACAAGGCATTTTATGAAAAACTCATTCGCATTGCAGATGATGAATTTACCCG
GCATCTGACAAGCCTGGAGACAGCCAAGTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175705 NZ_CP062721:3227865-3227923 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin