Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4513074..4513768 Replicon chromosome
Accession NZ_CP062719
Organism Escherichia coli O157:H7 strain Z1816

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag ILQ55_RS22850 Protein ID WP_001263495.1
Coordinates 4513074..4513472 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag ILQ55_RS22855 Protein ID WP_000554757.1
Coordinates 4513475..4513768 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4508661..4508741 - 81 NuclAT_11 - -
- 4508661..4508741 - 81 NuclAT_11 - -
- 4508661..4508741 - 81 NuclAT_11 - -
- 4508661..4508741 - 81 NuclAT_11 - -
ILQ55_RS22825 4509337..4509795 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
ILQ55_RS22830 4510056..4511513 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
ILQ55_RS22835 4511570..4512091 - 522 Protein_4470 peptide chain release factor H -
ILQ55_RS22840 4512090..4512293 - 204 Protein_4471 RNA ligase RtcB family protein -
ILQ55_RS22845 4512612..4513064 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
ILQ55_RS22850 4513074..4513472 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ILQ55_RS22855 4513475..4513768 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ILQ55_RS22860 4513820..4514875 - 1056 WP_001226186.1 DNA polymerase IV -
ILQ55_RS22865 4514946..4515731 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
ILQ55_RS22870 4515703..4517415 + 1713 Protein_4477 flagellar biosynthesis protein FlhA -
ILQ55_RS22875 4517560..4518057 - 498 Protein_4478 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4474920..4519006 44086


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T175677 WP_001263495.1 NZ_CP062719:c4513472-4513074 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T175677 NZ_CP082129:224494-224601 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT175677 WP_000554757.1 NZ_CP062719:c4513768-4513475 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT175677 NZ_CP082129:c224446-224380 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References