Detailed information of TA system TA175669

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3226313..3226538
• Replicon: chromosome
• Accession: NZ_CP062719
• Organism: Escherichia coli O157:H7 strain Z1816

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ55_RS16430
• Protein ID: WP_000813258.1
• Coordinates: 3226313..3226468 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3226480..3226538 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ55_RS16380	3221316..3221747	-	432	WP_001302123.1	tellurite resistance protein	-
ILQ55_RS16395	3222198..3222911	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ55_RS16400	3223047..3223244	-	198	WP_000917763.1	hypothetical protein	-
ILQ55_RS16405	3223469..3224023	-	555	WP_000640035.1	DUF1133 family protein	-
ILQ55_RS16410	3224086..3224391	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ55_RS16415	3224404..3225453	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ55_RS16420	3225455..3225727	-	273	WP_000191872.1	hypothetical protein	-
ILQ55_RS16425	3225849..3226193	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ55_RS16430	3226313..3226468	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	3226480..3226538	+	59	-	-	Antitoxin
ILQ55_RS16435	3226759..3227316	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ILQ55_RS16440	3227318..3227536	-	219	WP_000683609.1	DUF4014 family protein	-
ILQ55_RS16445	3227664..3227975	-	312	WP_001289673.1	hypothetical protein	-
ILQ55_RS16450	3227968..3228195	-	228	WP_000699809.1	hypothetical protein	-
ILQ55_RS16455	3228192..3228473	-	282	WP_000603384.1	DNA-binding protein	-
ILQ55_RS16460	3228506..3229222	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ55_RS16465	3229256..3229717	-	462	WP_000139447.1	replication protein	-
ILQ55_RS16470	3229710..3230753	-	1044	WP_001262402.1	hypothetical protein	-
ILQ55_RS16475	3230822..3231247	-	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ55_RS16480	3231231..3231473	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	3181343..3288854	107511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175669 WP_000813258.1 NZ_CP062719:c3226468-3226313 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175669 NZ_CP082127:c4168842-4168555 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175669 NZ_CP062719:3226480-3226538 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin