Detailed information of TA system TA175641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4499663..4500357
• Replicon: chromosome
• Accession: NZ_CP062717
• Organism: Escherichia coli O157:H7 strain Z1825

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ56_RS22770
• Protein ID: WP_001263495.1
• Coordinates: 4499663..4500061 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ56_RS22775
• Protein ID: WP_000554757.1
• Coordinates: 4500064..4500357 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4495250..4495330	-	81	NuclAT_11	-	-
-	4495250..4495330	-	81	NuclAT_11	-	-
-	4495250..4495330	-	81	NuclAT_11	-	-
-	4495250..4495330	-	81	NuclAT_11	-	-
ILQ56_RS22745	4495926..4496384	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ56_RS22750	4496645..4498102	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ56_RS22755	4498159..4498680	-	522	Protein_4454	peptide chain release factor H	-
ILQ56_RS22760	4498679..4498882	-	204	Protein_4455	RNA ligase RtcB family protein	-
ILQ56_RS22765	4499201..4499653	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ56_RS22770	4499663..4500061	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ56_RS22775	4500064..4500357	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ56_RS22780	4500409..4501464	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ56_RS22785	4501535..4502320	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ56_RS22790	4502292..4504004	+	1713	Protein_4461	flagellar biosynthesis protein FlhA	-
ILQ56_RS22795	4504149..4504646	-	498	Protein_4462	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175641 WP_001263495.1 NZ_CP062717:c4500061-4499663 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175641 NZ_CP082120:c4280636-4280286 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175641 WP_000554757.1 NZ_CP062717:c4500357-4500064 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175641 NZ_CP082120:c4280881-4280630 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6