Detailed information of TA system TA175605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4477240..4477934
• Replicon: chromosome
• Accession: NZ_CP062715
• Organism: Escherichia coli O157:H7 strain Z1826

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ57_RS22590
• Protein ID: WP_001263495.1
• Coordinates: 4477240..4477638 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ57_RS22595
• Protein ID: WP_000554757.1
• Coordinates: 4477641..4477934 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4472827..4472907	-	81	NuclAT_11	-	-
-	4472827..4472907	-	81	NuclAT_11	-	-
-	4472827..4472907	-	81	NuclAT_11	-	-
-	4472827..4472907	-	81	NuclAT_11	-	-
ILQ57_RS22565	4473503..4473961	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ57_RS22570	4474222..4475679	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ57_RS22575	4475736..4476257	-	522	Protein_4419	peptide chain release factor H	-
ILQ57_RS22580	4476256..4476459	-	204	Protein_4420	RNA ligase RtcB family protein	-
ILQ57_RS22585	4476778..4477230	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ57_RS22590	4477240..4477638	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ57_RS22595	4477641..4477934	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ57_RS22600	4477986..4479041	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ57_RS22605	4479112..4479897	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ57_RS22610	4479869..4481581	+	1713	Protein_4426	flagellar biosynthesis protein FlhA	-
ILQ57_RS22615	4481726..4482223	-	498	Protein_4427	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175605 WP_001263495.1 NZ_CP062715:c4477638-4477240 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175605 NZ_CP082037:c5076937-5076494 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175605 WP_000554757.1 NZ_CP062715:c4477934-4477641 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175605 NZ_CP082037:c5077164-5076934 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6