Detailed information of TA system TA175569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4473864..4474558
• Replicon: chromosome
• Accession: NZ_CP062713
• Organism: Escherichia coli O157:H7 strain Z1830

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ58_RS22560
• Protein ID: WP_001263495.1
• Coordinates: 4473864..4474262 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ58_RS22565
• Protein ID: WP_000554757.1
• Coordinates: 4474265..4474558 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4469451..4469531	-	81	NuclAT_11	-	-
-	4469451..4469531	-	81	NuclAT_11	-	-
-	4469451..4469531	-	81	NuclAT_11	-	-
-	4469451..4469531	-	81	NuclAT_11	-	-
ILQ58_RS22535	4470127..4470585	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ58_RS22540	4470846..4472303	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ58_RS22545	4472360..4472881	-	522	Protein_4413	peptide chain release factor H	-
ILQ58_RS22550	4472880..4473083	-	204	Protein_4414	RNA ligase RtcB family protein	-
ILQ58_RS22555	4473402..4473854	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ58_RS22560	4473864..4474262	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ58_RS22565	4474265..4474558	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ58_RS22570	4474610..4475665	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ58_RS22575	4475736..4476521	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ58_RS22580	4476493..4478205	+	1713	Protein_4420	flagellar biosynthesis protein FlhA	-
ILQ58_RS22585	4478350..4478847	-	498	Protein_4421	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175569 WP_001263495.1 NZ_CP062713:c4474262-4473864 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175569 NZ_CP082029:4192256-4192474 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175569 WP_000554757.1 NZ_CP062713:c4474558-4474265 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175569 NZ_CP082029:4191855-4192229 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: S1FJN6