Detailed information of TA system TA175564

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3586022..3586247
• Replicon: chromosome
• Accession: NZ_CP062713
• Organism: Escherichia coli O157:H7 strain Z1830

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ58_RS18435
• Protein ID: WP_000813263.1
• Coordinates: 3586022..3586177 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3586189..3586247 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ58_RS18400	3581476..3582189	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ58_RS18405	3582327..3582523	-	197	Protein_3602	TrmB family transcriptional regulator	-
ILQ58_RS18410	3582810..3583628	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ58_RS18415	3583780..3584151	-	372	WP_000090264.1	antitermination protein	-
ILQ58_RS18420	3584141..3584512	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ58_RS18425	3584525..3585574	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ58_RS18430	3585576..3585854	-	279	WP_001341388.1	hypothetical protein	-
ILQ58_RS18435	3586022..3586177	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3586189..3586247	+	59	-	-	Antitoxin
ILQ58_RS18440	3586782..3587555	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ58_RS18445	3587907..3588320	-	414	WP_001151233.1	DUF977 family protein	-
ILQ58_RS18450	3588336..3589106	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ58_RS18455	3589128..3589874	-	747	WP_000788751.1	ATP-binding protein	-
ILQ58_RS18460	3589881..3590972	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T175564 WP_000813263.1 NZ_CP062713:c3586177-3586022 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T175564 NZ_CP082029:867710-868093 [Klebsiella pneumoniae]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175564 NZ_CP062713:3586189-3586247 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin