Detailed information of TA system TA175559

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2693523..2693748
• Replicon: chromosome
• Accession: NZ_CP062713
• Organism: Escherichia coli O157:H7 strain Z1830

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ58_RS13725
• Protein ID: WP_000813258.1
• Coordinates: 2693593..2693748 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2693523..2693581 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ58_RS13675	2688588..2688830	+	243	WP_000747948.1	hypothetical protein	-
ILQ58_RS13680	2688814..2689239	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ58_RS13685	2689308..2690351	+	1044	WP_001262402.1	hypothetical protein	-
ILQ58_RS13690	2690344..2690805	+	462	WP_000139447.1	replication protein	-
ILQ58_RS13695	2690839..2691555	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ58_RS13700	2691588..2691869	+	282	WP_000603384.1	DNA-binding protein	-
ILQ58_RS13705	2691866..2692093	+	228	WP_000699809.1	hypothetical protein	-
ILQ58_RS13710	2692086..2692397	+	312	WP_001289673.1	hypothetical protein	-
ILQ58_RS13715	2692525..2692743	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ58_RS13720	2692745..2693302	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2693523..2693581	-	59	-	-	Antitoxin
ILQ58_RS13725	2693593..2693748	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ58_RS13730	2693868..2694212	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ58_RS13735	2694334..2694606	+	273	WP_000191872.1	hypothetical protein	-
ILQ58_RS13740	2694608..2695657	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ58_RS13745	2695670..2695975	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ58_RS13750	2696038..2696592	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ58_RS13755	2696817..2697014	+	198	WP_000917763.1	hypothetical protein	-
ILQ58_RS13760	2697150..2697863	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ58_RS13775	2698314..2698745	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7' / nleC	2631849..2738568	106719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175559 WP_000813258.1 NZ_CP062713:2693593-2693748 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175559 NZ_CP082028:c5185-4871 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175559 NZ_CP062713:c2693581-2693523 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin