Detailed information of TA system TA175495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4475193..4475887
• Replicon: chromosome
• Accession: NZ_CP062708
• Organism: Escherichia coli O157:H7 strain Z1832

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ60_RS22565
• Protein ID: WP_001263495.1
• Coordinates: 4475193..4475591 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ60_RS22570
• Protein ID: WP_000554757.1
• Coordinates: 4475594..4475887 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4470780..4470860	-	81	NuclAT_11	-	-
-	4470780..4470860	-	81	NuclAT_11	-	-
-	4470780..4470860	-	81	NuclAT_11	-	-
-	4470780..4470860	-	81	NuclAT_11	-	-
ILQ60_RS22540	4471456..4471914	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ60_RS22545	4472175..4473632	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ60_RS22550	4473689..4474210	-	522	Protein_4414	peptide chain release factor H	-
ILQ60_RS22555	4474209..4474412	-	204	Protein_4415	RNA ligase RtcB family protein	-
ILQ60_RS22560	4474731..4475183	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ60_RS22565	4475193..4475591	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ60_RS22570	4475594..4475887	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ60_RS22575	4475939..4476994	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ60_RS22580	4477065..4477850	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ60_RS22585	4477822..4479534	+	1713	Protein_4421	flagellar biosynthesis protein FlhA	-
ILQ60_RS22590	4479679..4480176	-	498	Protein_4422	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175495 WP_001263495.1 NZ_CP062708:c4475591-4475193 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175495 NZ_CP082010:5059118-5059405 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175495 WP_000554757.1 NZ_CP062708:c4475887-4475594 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175495 NZ_CP082010:5058879-5059118 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6