Detailed information of TA system TA175490

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3587335..3587560
• Replicon: chromosome
• Accession: NZ_CP062708
• Organism: Escherichia coli O157:H7 strain Z1832

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ60_RS18440
• Protein ID: WP_000813263.1
• Coordinates: 3587335..3587490 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3587502..3587560 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ60_RS18405	3582789..3583502	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ60_RS18410	3583640..3583836	-	197	Protein_3603	TrmB family transcriptional regulator	-
ILQ60_RS18415	3584123..3584941	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ60_RS18420	3585093..3585464	-	372	WP_000090264.1	antitermination protein	-
ILQ60_RS18425	3585454..3585825	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ60_RS18430	3585838..3586887	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ60_RS18435	3586889..3587167	-	279	WP_001341388.1	hypothetical protein	-
ILQ60_RS18440	3587335..3587490	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3587502..3587560	+	59	-	-	Antitoxin
ILQ60_RS18445	3588095..3588868	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ60_RS18450	3589220..3589633	-	414	WP_001151233.1	DUF977 family protein	-
ILQ60_RS18455	3589649..3590419	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ60_RS18460	3590441..3591187	-	747	WP_000788751.1	ATP-binding protein	-
ILQ60_RS18465	3591194..3592285	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T175490 WP_000813263.1 NZ_CP062708:c3587490-3587335 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T175490 NZ_CP082010:2070105-2070207 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 59 bp

>AT175490 NZ_CP062708:3587502-3587560 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin