Detailed information of TA system TA175457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4475178..4475872
• Replicon: chromosome
• Accession: NZ_CP062705
• Organism: Escherichia coli O157:H7 strain Z1833

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ61_RS22570
• Protein ID: WP_001263495.1
• Coordinates: 4475178..4475576 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ61_RS22575
• Protein ID: WP_000554757.1
• Coordinates: 4475579..4475872 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4470765..4470845	-	81	NuclAT_11	-	-
-	4470765..4470845	-	81	NuclAT_11	-	-
-	4470765..4470845	-	81	NuclAT_11	-	-
-	4470765..4470845	-	81	NuclAT_11	-	-
ILQ61_RS22545	4471441..4471899	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ61_RS22550	4472160..4473617	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ61_RS22555	4473674..4474195	-	522	Protein_4415	peptide chain release factor H	-
ILQ61_RS22560	4474194..4474397	-	204	Protein_4416	RNA ligase RtcB family protein	-
ILQ61_RS22565	4474716..4475168	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ61_RS22570	4475178..4475576	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ61_RS22575	4475579..4475872	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ61_RS22580	4475924..4476979	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ61_RS22585	4477050..4477835	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ61_RS22590	4477807..4479519	+	1713	Protein_4422	flagellar biosynthesis protein FlhA	-
ILQ61_RS22595	4479664..4480161	-	498	Protein_4423	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175457 WP_001263495.1 NZ_CP062705:c4475576-4475178 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175457 NZ_CP082001:875974-876381 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175457 WP_000554757.1 NZ_CP062705:c4475872-4475579 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175457 NZ_CP082001:875727-875993 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6