Detailed information of TA system TA175447

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2694840..2695065
• Replicon: chromosome
• Accession: NZ_CP062705
• Organism: Escherichia coli O157:H7 strain Z1833

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ61_RS13730
• Protein ID: WP_000813258.1
• Coordinates: 2694910..2695065 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2694840..2694898 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ61_RS13680	2689905..2690147	+	243	WP_000747948.1	hypothetical protein	-
ILQ61_RS13685	2690131..2690556	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ61_RS13690	2690625..2691668	+	1044	WP_001262402.1	hypothetical protein	-
ILQ61_RS13695	2691661..2692122	+	462	WP_000139447.1	replication protein	-
ILQ61_RS13700	2692156..2692872	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ61_RS13705	2692905..2693186	+	282	WP_000603384.1	DNA-binding protein	-
ILQ61_RS13710	2693183..2693410	+	228	WP_000699809.1	hypothetical protein	-
ILQ61_RS13715	2693403..2693714	+	312	WP_001289673.1	hypothetical protein	-
ILQ61_RS13720	2693842..2694060	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ61_RS13725	2694062..2694619	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2694840..2694898	-	59	-	-	Antitoxin
ILQ61_RS13730	2694910..2695065	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ61_RS13735	2695185..2695529	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ61_RS13740	2695651..2695923	+	273	WP_000191872.1	hypothetical protein	-
ILQ61_RS13745	2695925..2696974	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ61_RS13750	2696987..2697292	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ61_RS13755	2697355..2697909	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ61_RS13760	2698134..2698331	+	198	WP_000917763.1	hypothetical protein	-
ILQ61_RS13765	2698467..2699180	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ61_RS13780	2699631..2700062	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7' / nleC	2625447..2739885	114438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175447 WP_000813258.1 NZ_CP062705:2694910-2695065 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175447 NZ_CP081987:c2124767-2124498 [Shinella oryzae]
TTGGCCTGGGCGATTGAGATCACCGAAGGGGCTAAGAAAGAGCTCGATAAACTCGGTCATGTCGAGGCAAAGCGCATTCG
TAATTTTCTTGTCACGAAGCTTGCTTCCTCGGAAAATCCTCGTCTGCTGGGCAGCGCTTTGCAGGGCGCGCGGCTGGGAA
GCTATTGGCGGTATCGCGTTGGCGACTATCGGATCATTTGTGACATCCAGGACAACAGGCTTGTCGTGGTCGTCGTTCGT
GTAGGACATCGGCGTGAAATATATTCCTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175447 NZ_CP062705:c2694898-2694840 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin