Detailed information of TA system TA175435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 709720..710519
• Replicon: chromosome
• Accession: NZ_CP062705
• Organism: Escherichia coli O157:H7 strain Z1833

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: ILQ61_RS03465
• Protein ID: WP_000347273.1
• Coordinates: 709720..710184 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: ILQ61_RS03470
• Protein ID: WP_001302819.1
• Coordinates: 710184..710519 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T175435 WP_000347273.1 NZ_CP062705:c710184-709720 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T175435 NZ_CP081945:c5646-5350 [Pseudomonas sp. DR208]
ATGAAAGAACCAATTTACGAATACGATCCAGCGGAATTACTCACTGACGCGGAATCTATCGCCATCTTTATGGCTGATGC
TTTCGAATCTGGTGATGCCTCATTTATCGCGCAGGCGCTGAGCGTGGTAGCTCGCGCCAAAGGTATGACCGCGATTGCAA
AAGAGGCTGGCGTCACACGCGCACACCTCTACCAACTGAAAAACGGCAACCCAACGCTGAAAACCATGATCGGCGTGATG
CGTGCGGTTGGCCTGGACATGACGGCAAAACAGCACGTCGACGTAGCCCACGGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT175435 WP_001302819.1 NZ_CP062705:c710519-710184 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT175435 NZ_CP081945:c5945-5643 [Pseudomonas sp. DR208]
ATGAAAACGATTATCCAAACCGCCACTTACATGACTTGGGAACGCAAACTGCGTGACAAGAAGGCCAAACTGATTATCGC
GGCGCGGGTGTTGCGCGTAGCCCACGGCTTGCTTGGTGACGTGCAGCCAGTCGGTCAAGGCGTCAGTGAGTTGCGTATTC
ATCATGGCCCCGGCTACCGGGTGTATTTCCAGCAGCGCGGCGATCAGCTGGTCCTATTGCTCTGCGGTGGGGATAAAAGC
AGTCAGGCGCGGGATATAGAAACCGCAAAAACTCTGGCAAGCCAGTGGAGTGAAGACGAATGA

Structures

Toxin

• AlphaFold DB: V0SSH7