Detailed information of TA system TA175420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4409144..4409838
• Replicon: chromosome
• Accession: NZ_CP062702
• Organism: Escherichia coli O157:H7 strain Z1834

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ62_RS22040
• Protein ID: WP_001263495.1
• Coordinates: 4409144..4409542 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ62_RS22045
• Protein ID: WP_000554757.1
• Coordinates: 4409545..4409838 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4404731..4404811	-	81	NuclAT_11	-	-
-	4404731..4404811	-	81	NuclAT_11	-	-
-	4404731..4404811	-	81	NuclAT_11	-	-
-	4404731..4404811	-	81	NuclAT_11	-	-
ILQ62_RS22015	4405407..4405865	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ62_RS22020	4406126..4407583	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ62_RS22025	4407640..4408161	-	522	Protein_4314	peptide chain release factor H	-
ILQ62_RS22030	4408160..4408363	-	204	Protein_4315	RNA ligase RtcB family protein	-
ILQ62_RS22035	4408682..4409134	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ62_RS22040	4409144..4409542	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ62_RS22045	4409545..4409838	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ62_RS22050	4409890..4410945	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ62_RS22055	4411016..4411801	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ62_RS22060	4411773..4413485	+	1713	Protein_4321	flagellar biosynthesis protein FlhA	-
ILQ62_RS22065	4413630..4414127	-	498	Protein_4322	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175420 WP_001263495.1 NZ_CP062702:c4409542-4409144 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175420 NZ_CP081939:c1036341-1036063 [Avibacterium paragallinarum]
ATGATTACTCACTTTACTTGTAAAGATACCCAAGCCTTTTTTGAAGGGCAACGCATTCGCCGCTTTATTCCATTTGAAAA
AGTCGCAATGCGAAAATTACAGCAACTTAATGCCGCAACAGAACTCGATTTTTTAAGGATTCCACCAGGGAATCATTTAG
AAATGCTGACTGGTGATAGAAAAGGACAATACAGTATTAGAATTAACGATCAATGGCGAATCTGCTTTACTTGGTTAAAC
GGACACGCTGCCGATGTAGCAATTGTGGATTACCACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175420 WP_000554757.1 NZ_CP062702:c4409838-4409545 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175420 NZ_CP081939:c1036053-1035757 [Avibacterium paragallinarum]
ATGACAAGACAAATTCCCCTTTCTCACCCTGGCGAAATTTTGCTAGAAGAATGGCTAAAACCCTTAGGTATCAGCCAATA
TGCGTTAGCAAAAGCAATTTCTGTACCACCACGCCGAATCAATGAAATTGTCAAAGGAAAACGTGCGATCACACCAGACA
CGGCTTTACGCTTAGCCACCTATTTTGGGACAGATCCTCAAAGTTGGCTTAATCTCCAATCTCATTATGATACCGAGCAA
ACTCGTACCGCCATAAATGAAGATTTAACCCATATTTCTCCTTTTCAATTTGCCTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6