Detailed information of TA system TA175414

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3468861..3469086
• Replicon: chromosome
• Accession: NZ_CP062702
• Organism: Escherichia coli O157:H7 strain Z1834

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ62_RS17730
• Protein ID: WP_000813263.1
• Coordinates: 3468861..3469016 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3469028..3469086 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ62_RS17695	3464315..3465028	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ62_RS17700	3465166..3465362	-	197	Protein_3466	TrmB family transcriptional regulator	-
ILQ62_RS17705	3465649..3466467	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ62_RS17710	3466619..3466990	-	372	WP_000090264.1	antitermination protein	-
ILQ62_RS17715	3466980..3467351	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ62_RS17720	3467364..3468413	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ62_RS17725	3468415..3468693	-	279	WP_001341388.1	hypothetical protein	-
ILQ62_RS17730	3468861..3469016	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3469028..3469086	+	59	-	-	Antitoxin
ILQ62_RS17735	3469621..3470394	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ62_RS17740	3470746..3471159	-	414	WP_001151233.1	DUF977 family protein	-
ILQ62_RS17745	3471175..3471945	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ62_RS17750	3471967..3472713	-	747	WP_000788751.1	ATP-binding protein	-
ILQ62_RS17755	3472720..3473811	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T175414 WP_000813263.1 NZ_CP062702:c3469016-3468861 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T175414 NZ_CP081903:6256294-6256563 [Bacteroides cellulosilyticus]
ATGAAAGTAATATTTTCACCTCTTGCTATGGAGCAATGGGAGTACTGGAAGAAAAACAATCCTAATATTGCCAAACGCAT
CAAGAAGATTCTTCTCGACATTAGAGAGCATCCTTATACAGGAATCGCAAAGCCAGAACCTCTTAAATATGATTTAGCGG
GTAAATGGTCAAGACGCATCAATGAGGAACATAGGATTATTTACTCTGTAAATGATGACAGAATAGAGATTGATATACTA
TCAATGAGATACCATTACTCCAAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175414 NZ_CP062702:3469028-3469086 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin