Detailed information of TA system TA175410

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2622374..2622599
• Replicon: chromosome
• Accession: NZ_CP062702
• Organism: Escherichia coli O157:H7 strain Z1834

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ62_RS13230
• Protein ID: WP_000813258.1
• Coordinates: 2622444..2622599 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2622374..2622432 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ62_RS13180	2617439..2617681	+	243	WP_000747948.1	hypothetical protein	-
ILQ62_RS13185	2617665..2618090	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ62_RS13190	2618159..2619202	+	1044	WP_001262402.1	hypothetical protein	-
ILQ62_RS13195	2619195..2619656	+	462	WP_000139447.1	replication protein	-
ILQ62_RS13200	2619690..2620406	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ62_RS13205	2620439..2620720	+	282	WP_000603384.1	DNA-binding protein	-
ILQ62_RS13210	2620717..2620944	+	228	WP_000699809.1	hypothetical protein	-
ILQ62_RS13215	2620937..2621248	+	312	WP_001289673.1	hypothetical protein	-
ILQ62_RS13220	2621376..2621594	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ62_RS13225	2621596..2622153	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2622374..2622432	-	59	-	-	Antitoxin
ILQ62_RS13230	2622444..2622599	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ62_RS13235	2622719..2623063	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ62_RS13240	2623185..2623457	+	273	WP_000191872.1	hypothetical protein	-
ILQ62_RS13245	2623459..2624508	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ62_RS13250	2624521..2624826	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ62_RS13255	2624889..2625443	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ62_RS13260	2625668..2625865	+	198	WP_000917763.1	hypothetical protein	-
ILQ62_RS13265	2626001..2626714	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ62_RS13280	2627165..2627596	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / nleH2 / nleF	2549687..2659708	110021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175410 WP_000813258.1 NZ_CP062702:2622444-2622599 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175410 NZ_CP081897:c4572781-4572248 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTATTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175410 NZ_CP062702:c2622432-2622374 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin