Detailed information of TA system TA175383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4448003..4448697
• Replicon: chromosome
• Accession: NZ_CP062700
• Organism: Escherichia coli O157:H7 strain Z1835

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ63_RS22500
• Protein ID: WP_001263495.1
• Coordinates: 4448003..4448401 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ63_RS22505
• Protein ID: WP_000554757.1
• Coordinates: 4448404..4448697 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4443590..4443670	-	81	NuclAT_11	-	-
-	4443590..4443670	-	81	NuclAT_11	-	-
-	4443590..4443670	-	81	NuclAT_11	-	-
-	4443590..4443670	-	81	NuclAT_11	-	-
ILQ63_RS22475	4444266..4444724	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ63_RS22480	4444985..4446442	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ63_RS22485	4446499..4447020	-	522	Protein_4406	peptide chain release factor H	-
ILQ63_RS22490	4447019..4447222	-	204	Protein_4407	RNA ligase RtcB family protein	-
ILQ63_RS22495	4447541..4447993	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ63_RS22500	4448003..4448401	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ63_RS22505	4448404..4448697	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ63_RS22510	4448749..4449804	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ63_RS22515	4449875..4450660	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ63_RS22520	4450632..4452344	+	1713	Protein_4413	flagellar biosynthesis protein FlhA	-
ILQ63_RS22525	4452489..4452986	-	498	Protein_4414	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175383 WP_001263495.1 NZ_CP062700:c4448401-4448003 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175383 NZ_CP081895:726665-727153 [Klebsiella quasipneumoniae]
ATGATCTCCGCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTAAAACTGCGGGCGATGAAAAATCAGACCACTGGCGCGTCTCGTACCTTTGTGTGTTGTGGCAGCGATTCTAACGTGC
TGGCTTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCTCCCGGTCGCTTTCGCCGCAATATGCCTGACCCG
ATCCCTGTGGTGGTGCTGGGCCGTCTGGCGGTGGATCGGTCGCTACATGGACAAGGTGTTGGGCGGGCGCTGGTGAGGGA
TGCCGGACTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGTGGGATGTTGGTCCATGCCTTGTCGGATGAAGCGC
GGGAGTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACATTGGGGGATTTGGTT
CATGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175383 WP_000554757.1 NZ_CP062700:c4448697-4448404 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175383 NZ_CP081895:726375-726668 [Klebsiella quasipneumoniae]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACTCTGAATCTGCGCATCAAGCCCGCCGAGCGCGATCTTATCGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGTTGA
TTGAGCAACGCATTATTTTGGCCGATCCACAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGACTCCTGCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6