Detailed information of TA system TA175378

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3496889..3497114
• Replicon: chromosome
• Accession: NZ_CP062700
• Organism: Escherichia coli O157:H7 strain Z1835

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ63_RS17895
• Protein ID: WP_000813263.1
• Coordinates: 3496889..3497044 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3497056..3497114 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ63_RS17860	3492343..3493056	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ63_RS17865	3493194..3493390	-	197	Protein_3500	TrmB family transcriptional regulator	-
ILQ63_RS17870	3493677..3494495	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ63_RS17875	3494647..3495018	-	372	WP_000090264.1	antitermination protein	-
ILQ63_RS17880	3495008..3495379	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ63_RS17885	3495392..3496441	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ63_RS17890	3496443..3496721	-	279	WP_001341388.1	hypothetical protein	-
ILQ63_RS17895	3496889..3497044	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3497056..3497114	+	59	-	-	Antitoxin
ILQ63_RS17900	3497649..3498422	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ63_RS17905	3498774..3499187	-	414	WP_001151233.1	DUF977 family protein	-
ILQ63_RS17910	3499203..3499973	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ63_RS17915	3499995..3500741	-	747	WP_000788751.1	ATP-binding protein	-
ILQ63_RS17920	3500748..3501857	-	1110	WP_021499850.1	putative primosomal protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T175378 WP_000813263.1 NZ_CP062700:c3497044-3496889 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T175378 NZ_CP081894:4016667-4016885 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175378 NZ_CP062700:3497056-3497114 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin