Detailed information of TA system TA175375

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2928778..2929003
• Replicon: chromosome
• Accession: NZ_CP062700
• Organism: Escherichia coli O157:H7 strain Z1835

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ63_RS14695
• Protein ID: WP_000813258.1
• Coordinates: 2928778..2928933 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2928945..2929003 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ63_RS14645	2923781..2924212	-	432	WP_001302123.1	tellurite resistance protein	-
ILQ63_RS14660	2924663..2925376	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ63_RS14665	2925512..2925709	-	198	WP_000917763.1	hypothetical protein	-
ILQ63_RS14670	2925934..2926488	-	555	WP_000640035.1	DUF1133 family protein	-
ILQ63_RS14675	2926551..2926856	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ63_RS14680	2926869..2927918	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ63_RS14685	2927920..2928192	-	273	WP_000191872.1	hypothetical protein	-
ILQ63_RS14690	2928314..2928658	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ63_RS14695	2928778..2928933	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2928945..2929003	+	59	-	-	Antitoxin
ILQ63_RS14700	2929224..2929781	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ILQ63_RS14705	2929783..2930001	-	219	WP_000683609.1	DUF4014 family protein	-
ILQ63_RS14710	2930129..2930440	-	312	WP_001289673.1	hypothetical protein	-
ILQ63_RS14715	2930433..2930660	-	228	WP_000699809.1	hypothetical protein	-
ILQ63_RS14720	2930657..2930938	-	282	WP_000603384.1	DNA-binding protein	-
ILQ63_RS14725	2930971..2931646	-	676	Protein_2884	DUF1627 domain-containing protein	-
ILQ63_RS14730	2931680..2932141	-	462	WP_000139447.1	replication protein	-
ILQ63_RS14735	2932134..2933177	-	1044	WP_001262402.1	hypothetical protein	-
ILQ63_RS14740	2933246..2933671	-	426	WP_206356580.1	Rha family transcriptional regulator	-
ILQ63_RS14745	2933655..2933897	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / paa	2891674..2993838	102164

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175375 WP_000813258.1 NZ_CP062700:c2928933-2928778 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175375 NZ_CP081894:795189-795599 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175375 NZ_CP062700:2928945-2929003 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin