Detailed information of TA system TA175372

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2561364..2561589
• Replicon: chromosome
• Accession: NZ_CP062700
• Organism: Escherichia coli O157:H7 strain Z1835

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: ILQ63_RS12905
• Protein ID: WP_000813254.1
• Coordinates: 2561434..2561589 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2561364..2561422 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ63_RS12860	2556617..2557570	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
ILQ63_RS12865	2557577..2558317	+	741	WP_206356613.1	ATP-binding protein	-
ILQ63_RS12870	2558347..2559117	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
ILQ63_RS12875	2559133..2559528	+	396	WP_001118161.1	DUF977 family protein	-
ILQ63_RS12880	2559585..2559941	+	357	WP_001302146.1	hypothetical protein	-
ILQ63_RS12885	2559990..2560202	+	213	WP_000063625.1	hypothetical protein	-
ILQ63_RS12890	2560238..2560609	+	372	WP_000137941.1	hypothetical protein	-
ILQ63_RS12895	2560606..2560968	+	363	WP_000610379.1	DUF551 domain-containing protein	-
ILQ63_RS12900	2561084..2561188	+	105	WP_001278450.1	hypothetical protein	-
-	2561364..2561422	-	59	-	-	Antitoxin
ILQ63_RS12905	2561434..2561589	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
ILQ63_RS12910	2561868..2562035	+	168	WP_000998188.1	hypothetical protein	-
ILQ63_RS12915	2562101..2562379	+	279	WP_001304183.1	hypothetical protein	-
ILQ63_RS12920	2562381..2563430	+	1050	WP_001302870.1	DUF968 domain-containing protein	-
ILQ63_RS12925	2563443..2563817	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ63_RS12930	2563814..2564635	+	822	WP_000762904.1	antitermination protein	-
ILQ63_RS12935	2564862..2565059	+	198	WP_000917735.1	hypothetical protein	-
ILQ63_RS12940	2565210..2566268	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI	2541269..2599496	58227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T175372 WP_000813254.1 NZ_CP062700:2561434-2561589 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T175372 NZ_CP081893:c4746461-4746222 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGCGTTTGATCCGCGTGCCTGGCGCGAATGGCAGAAGCTTGGCGAGACGATCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGCAGAATCCGCGAATTGCATCGGCCAGTCTGAGTGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGGGATAGTGTGGTGGTGGTTTACGTTATTGCCAGTGGCTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175372 NZ_CP062700:c2561422-2561364 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin