Detailed information of TA system TA175237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2324420..2324937
• Replicon: chromosome
• Accession: NZ_CP062421
• Organism: Staphylococcus aureus strain NAS_AN_003

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: IF693_RS11350
• Protein ID: WP_000113262.1
• Coordinates: 2324671..2324937 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: IF693_RS11345
• Protein ID: WP_000584499.1
• Coordinates: 2324420..2324671 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF693_RS11315 (2320118)	2320118..2320837	+	720	WP_000479576.1	amino acid ABC transporter permease	-
IF693_RS11320 (2320834)	2320834..2321565	+	732	WP_000615470.1	amino acid ABC transporter ATP-binding protein	-
IF693_RS11325 (2321662)	2321662..2322912	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
IF693_RS11330 (2323167)	2323167..2323529	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
IF693_RS11335 (2323548)	2323548..2324147	+	600	WP_000162813.1	DsbA family protein	-
IF693_RS11340 (2324154)	2324154..2324255	+	102	WP_001791744.1	hypothetical protein	-
IF693_RS11345 (2324420)	2324420..2324671	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IF693_RS11350 (2324671)	2324671..2324937	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
IF693_RS11355 (2324972)	2324972..2325043	+	72	Protein_2204	hypothetical protein	-
IF693_RS11360 (2325232)	2325232..2326782	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IF693_RS11365 (2326968)	2326968..2327435	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
IF693_RS11370 (2327519)	2327519..2327701	+	183	WP_000230294.1	hypothetical protein	-
IF693_RS11375 (2327918)	2327918..2328742	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
IF693_RS11380 (2328975)	2328975..2329511	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T175237 WP_000113262.1 NZ_CP062421:2324671-2324937 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T175237 NZ_CP081831:c413-147 [Klebsiella quasipneumoniae]
TTGGTCTGGACGATTAACTATTCCGAACGGGCGTTAAAATCGTTACGTAAGATGGACAAGCAGACTGCACGACGAATTTT
GGATTTTATGGACTTACGCATTGCTGTTGCAGAAGATCCCCGTCAGTCAGGGAAACCGCTCAAAGGTGAGTTAGGTGAAT
TCTGGCGTTATCGGGTAGGCGATTATCGTATTCTGTGTGAAATCCTAGATGATGAGCTGGTCATCCTTGCGGCTACAATC
GGCCACCGACGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT175237 WP_000584499.1 NZ_CP062421:2324420-2324671 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT175237 NZ_CP081831:c621-397 [Klebsiella quasipneumoniae]
ATGTTGGCTATCCGGCTGTCTGCTGAAATTGAGTCTCGTCTTGATTTACTGGCGAAAAAAACCGGCAGAACAAAGACGTT
CTATGCGCGGGAAGCGATACTTGCACACTTGGAAGATCTGGAAGATTACTATCTTTCAGCAGAAACTGCCGCACGCGTTC
GGCGTGGTGATGAGGCTGTGCATTCGTCTGAAGCCGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: T1YB60