Detailed information of TA system TA175178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2321652..2322169
• Replicon: chromosome
• Accession: NZ_CP062417
• Organism: Staphylococcus aureus strain NAS_AN_143

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: IF718_RS11305
• Protein ID: WP_000113265.1
• Coordinates: 2321903..2322169 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: IF718_RS11300
• Protein ID: WP_000584499.1
• Coordinates: 2321652..2321903 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF718_RS11270 (2317349)	2317349..2318068	+	720	WP_000479576.1	amino acid ABC transporter permease	-
IF718_RS11275 (2318065)	2318065..2318796	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
IF718_RS11280 (2318893)	2318893..2320143	-	1251	WP_240104165.1	FemA/FemB family glycyltransferase FmhA	-
IF718_RS11285 (2320399)	2320399..2320761	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
IF718_RS11290 (2320780)	2320780..2321379	+	600	WP_000162810.1	DsbA family protein	-
IF718_RS11295 (2321407)	2321407..2321487	+	81	WP_223197974.1	hypothetical protein	-
IF718_RS11300 (2321652)	2321652..2321903	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IF718_RS11305 (2321903)	2321903..2322169	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
IF718_RS11310 (2322204)	2322204..2322275	+	72	Protein_2198	hypothetical protein	-
IF718_RS11315 (2322464)	2322464..2324011	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IF718_RS11320 (2324197)	2324197..2324664	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
IF718_RS11325 (2324748)	2324748..2324930	+	183	WP_000230292.1	hypothetical protein	-
IF718_RS11330 (2325147)	2325147..2325971	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
IF718_RS11335 (2326205)	2326205..2326741	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T175178 WP_000113265.1 NZ_CP062417:2321903-2322169 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T175178 NZ_CP081791:3763999-3764340 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT175178 WP_000584499.1 NZ_CP062417:2321652-2321903 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT175178 NZ_CP081791:3763661-3763978 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60