Detailed information of TA system TA175124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2336701..2337218
• Replicon: chromosome
• Accession: NZ_CP062414
• Organism: Staphylococcus aureus strain NAS_AN_175

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: IF723_RS11385
• Protein ID: WP_000113262.1
• Coordinates: 2336952..2337218 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: IF723_RS11380
• Protein ID: WP_000584499.1
• Coordinates: 2336701..2336952 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF723_RS11350 (2332399)	2332399..2333118	+	720	WP_000479576.1	amino acid ABC transporter permease	-
IF723_RS11355 (2333115)	2333115..2333846	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
IF723_RS11360 (2333943)	2333943..2335193	-	1251	WP_001012228.1	FemA/FemB family glycyltransferase FmhA	-
IF723_RS11365 (2335448)	2335448..2335810	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
IF723_RS11370 (2335829)	2335829..2336428	+	600	WP_000162803.1	DsbA family protein	-
IF723_RS11375 (2336456)	2336456..2336536	+	81	WP_223197974.1	hypothetical protein	-
IF723_RS11380 (2336701)	2336701..2336952	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IF723_RS11385 (2336952)	2336952..2337218	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
IF723_RS11390 (2337253)	2337253..2337324	+	72	WP_223200558.1	hypothetical protein	-
IF723_RS11395 (2337512)	2337512..2339062	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IF723_RS11400 (2339248)	2339248..2339715	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
IF723_RS11405 (2339802)	2339802..2339984	+	183	WP_000079142.1	hypothetical protein	-
IF723_RS11410 (2340201)	2340201..2341025	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
IF723_RS11415 (2341259)	2341259..2341795	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T175124 WP_000113262.1 NZ_CP062414:2336952-2337218 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T175124 NZ_CP081774:c4150113-4149799 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT175124 WP_000584499.1 NZ_CP062414:2336701-2336952 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT175124 NZ_CP081774:c4150349-4150116 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: T1YB60