Detailed information of TA system TA175085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2336577..2337094
• Replicon: chromosome
• Accession: NZ_CP062412
• Organism: Staphylococcus aureus strain NAS_AN_181

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: IF725_RS11380
• Protein ID: WP_000113262.1
• Coordinates: 2336828..2337094 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: IF725_RS11375
• Protein ID: WP_000584499.1
• Coordinates: 2336577..2336828 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF725_RS11345 (2332275)	2332275..2332994	+	720	WP_000479576.1	amino acid ABC transporter permease	-
IF725_RS11350 (2332991)	2332991..2333722	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
IF725_RS11355 (2333819)	2333819..2335069	-	1251	WP_001012228.1	FemA/FemB family glycyltransferase FmhA	-
IF725_RS11360 (2335324)	2335324..2335686	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
IF725_RS11365 (2335705)	2335705..2336304	+	600	WP_000162803.1	DsbA family protein	-
IF725_RS11370 (2336332)	2336332..2336412	+	81	WP_223197974.1	hypothetical protein	-
IF725_RS11375 (2336577)	2336577..2336828	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IF725_RS11380 (2336828)	2336828..2337094	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
IF725_RS11385 (2337129)	2337129..2337200	+	72	WP_223200558.1	hypothetical protein	-
IF725_RS11390 (2337388)	2337388..2338938	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IF725_RS11395 (2339124)	2339124..2339591	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
IF725_RS11400 (2339678)	2339678..2339860	+	183	WP_000079142.1	hypothetical protein	-
IF725_RS11405 (2340077)	2340077..2340901	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
IF725_RS11410 (2341135)	2341135..2341671	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T175085 WP_000113262.1 NZ_CP062412:2336828-2337094 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T175085 NZ_CP081767:1046790-1047125 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAAGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT175085 WP_000584499.1 NZ_CP062412:2336577-2336828 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT175085 NZ_CP081767:1046542-1046790 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60