Detailed information of TA system TA175014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2416909..2417426
• Replicon: chromosome
• Accession: NZ_CP062408
• Organism: Staphylococcus aureus strain NAS_AN_247

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: IF735_RS11900
• Protein ID: WP_000113262.1
• Coordinates: 2417160..2417426 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: IF735_RS11895
• Protein ID: WP_000584499.1
• Coordinates: 2416909..2417160 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF735_RS11865 (2412607)	2412607..2413326	+	720	WP_000479576.1	amino acid ABC transporter permease	-
IF735_RS11870 (2413323)	2413323..2414054	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
IF735_RS11875 (2414151)	2414151..2415401	-	1251	WP_001012228.1	FemA/FemB family glycyltransferase FmhA	-
IF735_RS11880 (2415656)	2415656..2416018	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
IF735_RS11885 (2416037)	2416037..2416636	+	600	WP_000162803.1	DsbA family protein	-
IF735_RS11890 (2416664)	2416664..2416744	+	81	WP_223197974.1	hypothetical protein	-
IF735_RS11895 (2416909)	2416909..2417160	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IF735_RS11900 (2417160)	2417160..2417426	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
IF735_RS11905 (2417461)	2417461..2417532	+	72	WP_223200558.1	hypothetical protein	-
IF735_RS11910 (2417720)	2417720..2419270	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IF735_RS11915 (2419456)	2419456..2419923	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
IF735_RS11920 (2420010)	2420010..2420192	+	183	WP_000079142.1	hypothetical protein	-
IF735_RS11925 (2420409)	2420409..2421233	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
IF735_RS11930 (2421467)	2421467..2422003	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T175014 WP_000113262.1 NZ_CP062408:2417160-2417426 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T175014 NZ_CP081724:c4222777-4222490 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT175014 WP_000584499.1 NZ_CP062408:2416909-2417160 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT175014 NZ_CP081724:c4222503-4222201 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60