Detailed information of TA system TA174635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2501183..2501700
• Replicon: chromosome
• Accession: NZ_CP062366
• Organism: Staphylococcus aureus strain NAS_AN_123

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: IF713_RS12535
• Protein ID: WP_000113262.1
• Coordinates: 2501434..2501700 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A5A8VWS3
• Locus tag: IF713_RS12530
• Protein ID: WP_000584502.1
• Coordinates: 2501183..2501434 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF713_RS12500 (2496884)	2496884..2497603	+	720	WP_000479576.1	amino acid ABC transporter permease	-
IF713_RS12505 (2497600)	2497600..2498331	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
IF713_RS12510 (2498428)	2498428..2499678	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
IF713_RS12515 (2499933)	2499933..2500292	+	360	WP_000819847.1	DUF4467 domain-containing protein	-
IF713_RS12520 (2500311)	2500311..2500910	+	600	WP_000162813.1	DsbA family protein	-
IF713_RS12525 (2500917)	2500917..2501018	+	102	WP_001791744.1	hypothetical protein	-
IF713_RS12530 (2501183)	2501183..2501434	+	252	WP_000584502.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IF713_RS12535 (2501434)	2501434..2501700	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
IF713_RS12540 (2501735)	2501735..2501806	+	72	Protein_2444	hypothetical protein	-
IF713_RS12545 (2501995)	2501995..2503545	+	1551	WP_000727762.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IF713_RS12550 (2503731)	2503731..2504198	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
IF713_RS12555 (2504282)	2504282..2504464	+	183	WP_000230294.1	hypothetical protein	-
IF713_RS12560 (2504681)	2504681..2505505	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
IF713_RS12565 (2505738)	2505738..2506274	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T174635 WP_000113262.1 NZ_CP062366:2501434-2501700 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T174635 NZ_CP081306:1344771-1345169 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9417.65 Da
• Isoelectric Point: 4.4702

>AT174635 WP_000584502.1 NZ_CP062366:2501183-2501434 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT174635 NZ_CP081306:1344544-1344771 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: A0A5A8VWS3