Detailed information of TA system TA174506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2317552..2318069
• Replicon: chromosome
• Accession: NZ_CP062350
• Organism: Staphylococcus aureus strain NAS_AN_250

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: IF736_RS11430
• Protein ID: WP_000113262.1
• Coordinates: 2317803..2318069 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: IF736_RS11425
• Protein ID: WP_000584499.1
• Coordinates: 2317552..2317803 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF736_RS11395 (2313250)	2313250..2313969	+	720	WP_000479576.1	amino acid ABC transporter permease	-
IF736_RS11400 (2313966)	2313966..2314697	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
IF736_RS11405 (2314794)	2314794..2316044	-	1251	WP_001012223.1	FemA/FemB family glycyltransferase FmhA	-
IF736_RS11410 (2316299)	2316299..2316661	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
IF736_RS11415 (2316680)	2316680..2317279	+	600	WP_000162813.1	DsbA family protein	-
IF736_RS11420 (2317286)	2317286..2317387	+	102	WP_031774922.1	hypothetical protein	-
IF736_RS11425 (2317552)	2317552..2317803	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IF736_RS11430 (2317803)	2317803..2318069	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
IF736_RS11435 (2318104)	2318104..2318181	+	78	Protein_2224	hypothetical protein	-
IF736_RS11440 (2318365)	2318365..2319915	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IF736_RS11445 (2320101)	2320101..2320568	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
IF736_RS11450 (2320652)	2320652..2320834	+	183	WP_240018937.1	hypothetical protein	-
IF736_RS11455 (2321051)	2321051..2321875	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
IF736_RS11460 (2322108)	2322108..2322644	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T174506 WP_000113262.1 NZ_CP062350:2317803-2318069 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T174506 NZ_CP081047:c38793-38491 [Leisingera aquaemixtae]
ATGGCTGAGCCGCGCTGGCGGATCCGCCCCGCCGCCCGCGCCGACCTTGCCGCCATCTGGCGCTATGGCCGGGACACCTG
GGGCGCGGCGCAGGCAGACGCCTATACAGACAGCCTGTTTGCCCTGTTCGACCTGCTGGCGGACTTCCCCGAAATGGCCC
GCGAACGGGCTGAGTTCACCCCGCCGGTCCGCATCCACCCCTCAGGCGCGCATCTGGTGCTCTACCGGCTGGAGCACGGC
CGCCCCGAGATCCTCCGCATCCTGCACGCCCGCCAGGACCTGATGGCGTTCCTCTCGGAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT174506 WP_000584499.1 NZ_CP062350:2317552-2317803 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT174506 NZ_CP081047:c39022-38786 [Leisingera aquaemixtae]
ATGGCCACCATGAATGTATCTCTGCCGGACCAGATGAAGGACTGGGTCGAGGAACAGGCCCGCACCGGTACCTATGCCAA
TTCCAGCGACTATGTCCGCGACCTGATCCGCCGCGACCAGGCCCGCACCGCTGCCATTGCCGAGCTGCAATCCGCCATTG
ATGCAGGTCTCGCCAGCGGCCCGGCGGAACCGCTGAGCGCCGAAAGCTTCAAGGCCGCCATGCGCCGCAATGGCTGA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60