Detailed information of TA system TA174434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2374362..2374879
• Replicon: chromosome
• Accession: NZ_CP062342
• Organism: Staphylococcus aureus strain NAS_AN_270

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: IF741_RS11685
• Protein ID: WP_000113262.1
• Coordinates: 2374613..2374879 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: IF741_RS11680
• Protein ID: WP_000584499.1
• Coordinates: 2374362..2374613 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF741_RS11650 (2370060)	2370060..2370779	+	720	WP_000479576.1	amino acid ABC transporter permease	-
IF741_RS11655 (2370776)	2370776..2371507	+	732	WP_000615470.1	amino acid ABC transporter ATP-binding protein	-
IF741_RS11660 (2371604)	2371604..2372854	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
IF741_RS11665 (2373109)	2373109..2373471	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
IF741_RS11670 (2373490)	2373490..2374089	+	600	WP_000162813.1	DsbA family protein	-
IF741_RS11675 (2374096)	2374096..2374197	+	102	WP_001791744.1	hypothetical protein	-
IF741_RS11680 (2374362)	2374362..2374613	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IF741_RS11685 (2374613)	2374613..2374879	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
IF741_RS11690 (2374914)	2374914..2374985	+	72	Protein_2274	hypothetical protein	-
IF741_RS11695 (2375174)	2375174..2376724	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IF741_RS11700 (2376910)	2376910..2377377	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
IF741_RS11705 (2377461)	2377461..2377643	+	183	WP_000230294.1	hypothetical protein	-
IF741_RS11710 (2377860)	2377860..2378684	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
IF741_RS11715 (2378917)	2378917..2379453	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T174434 WP_000113262.1 NZ_CP062342:2374613-2374879 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T174434 NZ_CP081008:c3796658-3796404 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT174434 WP_000584499.1 NZ_CP062342:2374362-2374613 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT174434 NZ_CP081008:c3796906-3796655 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60