Detailed information of TA system TA174417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2364330..2364847
• Replicon: chromosome
• Accession: NZ_CP062340
• Organism: Staphylococcus aureus strain NAS_AN_275

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: IF742_RS11630
• Protein ID: WP_000113262.1
• Coordinates: 2364581..2364847 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: IF742_RS11625
• Protein ID: WP_000584499.1
• Coordinates: 2364330..2364581 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF742_RS11595 (2360028)	2360028..2360747	+	720	WP_000479576.1	amino acid ABC transporter permease	-
IF742_RS11600 (2360744)	2360744..2361475	+	732	WP_000615470.1	amino acid ABC transporter ATP-binding protein	-
IF742_RS11605 (2361572)	2361572..2362822	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
IF742_RS11610 (2363077)	2363077..2363439	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
IF742_RS11615 (2363458)	2363458..2364057	+	600	WP_000162813.1	DsbA family protein	-
IF742_RS11620 (2364064)	2364064..2364165	+	102	WP_001791744.1	hypothetical protein	-
IF742_RS11625 (2364330)	2364330..2364581	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IF742_RS11630 (2364581)	2364581..2364847	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
IF742_RS11635 (2364882)	2364882..2364953	+	72	Protein_2265	hypothetical protein	-
IF742_RS11640 (2365142)	2365142..2366692	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IF742_RS11645 (2366878)	2366878..2367345	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
IF742_RS11650 (2367429)	2367429..2367611	+	183	WP_000230294.1	hypothetical protein	-
IF742_RS11655 (2367828)	2367828..2368652	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
IF742_RS11660 (2368885)	2368885..2369421	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T174417 WP_000113262.1 NZ_CP062340:2364581-2364847 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T174417 NZ_CP081008:c1111325-1111107 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT174417 WP_000584499.1 NZ_CP062340:2364330-2364581 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT174417 NZ_CP081008:c1111725-1111351 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: T1YB60