Detailed information of TA system TA174366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2370107..2370624
• Replicon: chromosome
• Accession: NZ_CP062334
• Organism: Staphylococcus aureus strain NAS_NP_164

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: IF749_RS11680
• Protein ID: WP_000113262.1
• Coordinates: 2370358..2370624 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: IF749_RS11675
• Protein ID: WP_000584499.1
• Coordinates: 2370107..2370358 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF749_RS11645 (2365805)	2365805..2366524	+	720	WP_000479576.1	amino acid ABC transporter permease	-
IF749_RS11650 (2366521)	2366521..2367252	+	732	WP_000615470.1	amino acid ABC transporter ATP-binding protein	-
IF749_RS11655 (2367349)	2367349..2368599	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
IF749_RS11660 (2368854)	2368854..2369216	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
IF749_RS11665 (2369235)	2369235..2369834	+	600	WP_000162813.1	DsbA family protein	-
IF749_RS11670 (2369841)	2369841..2369942	+	102	WP_001791744.1	hypothetical protein	-
IF749_RS11675 (2370107)	2370107..2370358	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IF749_RS11680 (2370358)	2370358..2370624	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
IF749_RS11685 (2370659)	2370659..2370730	+	72	Protein_2273	hypothetical protein	-
IF749_RS11690 (2370919)	2370919..2372469	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IF749_RS11695 (2372655)	2372655..2373122	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
IF749_RS11700 (2373206)	2373206..2373388	+	183	WP_000230294.1	hypothetical protein	-
IF749_RS11705 (2373605)	2373605..2374429	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
IF749_RS11710 (2374662)	2374662..2375198	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T174366 WP_000113262.1 NZ_CP062334:2370358-2370624 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T174366 NZ_CP080999:c2264389-2264132 [Mycobacterium malmoense]
GTGAGAAGCATCCACTTCGATCCCAATGCCTGGGAGGACTTTCTATTCTGGCTGGGCTCCGACCGCAAGGCGGCCCGTCG
GGTCACCCGCCTGATCGCCGAGGTCCAACGCGATCCTTTCCGTGGCACCGGCAAACCCGAACCACTCAAGGGCGAGCTAT
CGGGTTACTGGTCCCGTCGAATCGACGACCAACATCGGCTTGTGTACCGAGCCGATGGCGACGAAGTCAAAATCCTCAAG
GCCCGATACCACTACTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT174366 WP_000584499.1 NZ_CP062334:2370107-2370358 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT174366 NZ_CP080999:c2264661-2264386 [Mycobacterium malmoense]
ATGAGCATCAGCGCAAGCGAGGCTAGACAACGCCTGTACCCGCTTCTCGCGCAAGTCAATACCGATCACCAACCTGTGCG
CATCACCTCCAAAGCCGGCGACGCGGTGCTCATGTCCGCCGACGACTACGACTCCTGGCAGGAAACGGTCTACCTGCTGC
GCTCACCAAAGAATGCCCAACGACTCATGGAAGCGGTCGCCCGGGACAAGGCCGGGCACTTTGCACTCACCAAGTCCGTC
GACGAGCTGCAGGAGATGGCCGGCGGCCAGGAGTGA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: T1YB60