Detailed information of TA system TA174247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2321736..2322253
• Replicon: chromosome
• Accession: NZ_CP062318
• Organism: Staphylococcus aureus strain NAS_OP_132

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: IF775_RS11315
• Protein ID: WP_000113262.1
• Coordinates: 2321987..2322253 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: IF775_RS11310
• Protein ID: WP_000584499.1
• Coordinates: 2321736..2321987 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF775_RS11280 (2317434)	2317434..2318153	+	720	WP_000479576.1	amino acid ABC transporter permease	-
IF775_RS11285 (2318150)	2318150..2318881	+	732	WP_000615470.1	amino acid ABC transporter ATP-binding protein	-
IF775_RS11290 (2318978)	2318978..2320228	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
IF775_RS11295 (2320483)	2320483..2320845	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
IF775_RS11300 (2320864)	2320864..2321463	+	600	WP_000162813.1	DsbA family protein	-
IF775_RS11305 (2321470)	2321470..2321571	+	102	WP_001791744.1	hypothetical protein	-
IF775_RS11310 (2321736)	2321736..2321987	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IF775_RS11315 (2321987)	2321987..2322253	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
IF775_RS11320 (2322288)	2322288..2322359	+	72	Protein_2202	hypothetical protein	-
IF775_RS11325 (2322548)	2322548..2324098	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IF775_RS11330 (2324284)	2324284..2324751	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
IF775_RS11335 (2324835)	2324835..2325017	+	183	WP_000230294.1	hypothetical protein	-
IF775_RS11340 (2325234)	2325234..2326058	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
IF775_RS11345 (2326291)	2326291..2326827	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T174247 WP_000113262.1 NZ_CP062318:2321987-2322253 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T174247 NZ_CP080599:c3144959-3144708 [Nocardiopsis sp. MT53]
TTGAAGACCATGAGCTACACCGAATCCCGCGCCAACTACGCAGCCACCCTCGACGCCGTCGTGAACGACAGAGAAGAAGT
GGTGATCACGAGAGCCGGACACGAACCCGTCGTCATGATGGCCCTGTCCGAGTACGAGACCCTGAAGGAGACCGCCTACC
TCCTGCGCAGCCCGGAGAACGCCCGCCGCCTGGTCTCCGCCATCGAGGAACTGGAGTCCGATCAGGGCATAGAGCGCGAG
TTGGCCGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT174247 WP_000584499.1 NZ_CP062318:2321736-2321987 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT174247 NZ_CP080599:c3144711-3144451 [Nocardiopsis sp. MT53]
GTGAGGCTCGTCTGGTCCCAGAGCGCCTGGGAGGACTACGTCTGGTGGCAGGGGCAGGACCGCAGGATCCTCAAGCGCAT
CAACGCTCTCGTCAAGGACATCGAGCGCAACGGCAACGAGGGCATGGGCAAGCCCGAACCCCTGAGGCACGGCTTCCAGG
GCTACTGGTCCCGCAGGATCACCGACGAGCACCGGCTGGTGTACAAGCTCGCCGGCGACGAGATCCGCATCGCCGCCTGC
CGCTACCACTACGGCAAGTAG

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: T1YB60