Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 213784..214319 Replicon plasmid pVB3338_P1
Accession NZ_CP062266
Organism Enterococcus faecium strain VB3338

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag IG632_RS14660 Protein ID WP_002314366.1
Coordinates 213784..214056 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag IG632_RS14665 Protein ID WP_002297422.1
Coordinates 214056..214319 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IG632_RS14620 208891..209340 - 450 WP_010729386.1 TlpA family protein disulfide reductase -
IG632_RS14625 209496..210038 - 543 WP_002298088.1 hypothetical protein -
IG632_RS14630 210313..210956 + 644 Protein_230 transposase -
IG632_RS14635 211032..211712 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
IG632_RS14640 211771..211959 - 189 Protein_232 hypothetical protein -
IG632_RS14645 211960..212817 - 858 WP_002292680.1 AAA family ATPase -
IG632_RS14650 213102..213388 - 287 Protein_234 type II toxin-antitoxin system RelE/ParE family toxin -
IG632_RS14655 213378..213707 - 330 WP_002292678.1 AbrB family transcriptional regulator -
IG632_RS14660 213784..214056 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
IG632_RS14665 214056..214319 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IG632_RS14670 214553..215461 + 909 Protein_238 IS982 family transposase -
IG632_RS14675 215464..216639 - 1176 WP_002336205.1 MFS transporter -
IG632_RS14680 216642..218891 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..227172 227172
- inside IScluster/Tn - - 202222..224920 22698


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T174193 WP_002314366.1 NZ_CP062266:c214056-213784 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T174193 NZ_CP080584:329422-329790 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT174193 WP_002297422.1 NZ_CP062266:c214319-214056 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT174193 NZ_CP080584:329204-329425 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References