Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 489553..490178 Replicon chromosome
Accession NZ_CP062254
Organism Pectobacterium parmentieri strain QK-5

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3FNM7
Locus tag IG623_RS02440 Protein ID WP_033070820.1
Coordinates 489553..489858 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IG623_RS02445 Protein ID WP_103808699.1
Coordinates 489855..490178 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IG623_RS02415 485275..485922 + 648 WP_012822190.1 type I-E CRISPR-associated protein Cas6/Cse3/CasE -
IG623_RS02420 485950..486867 + 918 WP_012822191.1 type I-E CRISPR-associated endonuclease Cas1 -
IG623_RS02425 486868..487161 + 294 WP_005973730.1 type I-E CRISPR-associated endoribonuclease Cas2 -
IG623_RS02430 488696..489067 - 372 WP_103808702.1 helix-turn-helix domain-containing protein -
IG623_RS02435 489141..489401 - 261 WP_012822193.1 type II toxin-antitoxin system RelE/ParE family toxin -
IG623_RS02440 489553..489858 + 306 WP_033070820.1 type II toxin-antitoxin system HigB family toxin Toxin
IG623_RS02445 489855..490178 + 324 WP_103808699.1 helix-turn-helix domain-containing protein Antitoxin
IG623_RS02450 491341..491799 + 459 WP_012822197.1 hypothetical protein -
IG623_RS02455 491880..493232 - 1353 WP_103808643.1 aspartate aminotransferase family protein -
IG623_RS02460 493263..494534 - 1272 WP_103808673.1 MFS transporter -
IG623_RS02465 494689..495135 + 447 WP_012822200.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(18-90)

Antitoxin

Cro/C1-type helix-turn-helix domain

(52-105)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 12010.78 Da        Isoelectric Point: 10.0386

>T174172 WP_033070820.1 NZ_CP062254:489553-489858 [Pectobacterium parmentieri]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITSHAEYGSLTEYYRRNKE

Download         Length: 306 bp

>T174172 NZ_CP080564:c2441623-2441516 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11655.44 Da        Isoelectric Point: 5.0877

>AT174172 WP_103808699.1 NZ_CP062254:489855-490178 [Pectobacterium parmentieri]
MMYADAIKAANNLTSIVPLLGGSTSCKDYEDAPEFVAFNERIKALPAGIALLRILMDQHGLTQSDFEAEIGKKSLVSRIL
NGERTLTVDHMSALAKRFDIPVSAFVD

Download         Length: 324 bp

>AT174172 NZ_CP080564:2441439-2441499 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3FNM7

Antitoxin

Source ID Structure

References