Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2083891..2084393 Replicon chromosome
Accession NZ_CP062249
Organism Escherichia coli strain AML002_ev01

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IHN46_RS10325 Protein ID WP_000767829.1
Coordinates 2083891..2084145 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IHN46_RS10330 Protein ID WP_001259255.1
Coordinates 2084142..2084393 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN46_RS10295 2078906..2079133 - 228 WP_000234896.1 sulfurtransferase TusA -
IHN46_RS10300 2079147..2080205 - 1059 WP_000492339.1 transport protein YeeE -
IHN46_RS10305 2080384..2081742 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IHN46_RS10310 2081732..2081794 - 63 WP_010723108.1 membrane protein YoeI -
IHN46_RS10315 2082009..2082938 - 930 WP_000803351.1 LysR family transcriptional regulator -
IHN46_RS10320 2082984..2083808 - 825 WP_000754737.1 SDR family oxidoreductase -
IHN46_RS10325 2083891..2084145 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IHN46_RS10330 2084142..2084393 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IHN46_RS10335 2084676..2084726 + 51 WP_001364200.1 his operon leader peptide -
IHN46_RS10340 2084872..2085771 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
IHN46_RS10345 2085777..2087081 + 1305 WP_000009594.1 histidinol dehydrogenase -
IHN46_RS10350 2087078..2088148 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
IHN46_RS10355 2088148..2089215 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T174086 WP_000767829.1 NZ_CP062249:c2084145-2083891 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T174086 NZ_CP080536:926648-926929 [Acidithiobacillus ferriphilus]
ATGATTCAAACATTCGCCAACACTGAAACCGAAGGCTTTTTCGTTACAGGTAAATCGCGTCGTTTACCTTCGGAAATTCT
CAAGCGTGCAGCCATGCGTCTCAATCAGTTGCATGCCGCTACAGAGTTGAATGATCTACATTTGCCACCCTCTAATCACT
TGGAGGCTCTTTCAGGTAATCGCATTGGTCAATGGAGTATACGTATAAACCAACAGTGGCGTGTCTGTTTCAAGTTTGAA
AATGGTGACGCATTTAATGTCGAAATAGTGGATTACCACTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT174086 WP_001259255.1 NZ_CP062249:c2084393-2084142 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT174086 NZ_CP080536:926940-927236 [Acidithiobacillus ferriphilus]
ATGATCTACAATGGTTTAACACCTATCCATCCCGGCGAGTACCTCGCCGAGCTCCTTGAAGAGCTTCATATCTCTCAAGC
GTTGTTCGCTCGTACCATTGGTGTGTCGCCCATGCGCATTTCCCATATCGTTAAAGGCAAGCGCCCTGTTACGGCTGAAC
TAGCATTGCTGTTTGCAAAAGCTTTTGAGCAAACTCCACAGTATTGGCTCAATTTACAAGCCAGCTATGATCTAAAAACT
GCTGAGAAAAGCATCGCCAATCGTCTCTTCTCCATTCATAGCTTGGCCCATGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References