Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3270362..3271161 Replicon chromosome
Accession NZ_CP062248
Organism Escherichia coli strain AML002_ev02

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag IHN47_RS15870 Protein ID WP_000347273.1
Coordinates 3270697..3271161 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IHN47_RS15865 Protein ID WP_001307405.1
Coordinates 3270362..3270697 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN47_RS15850 3266147..3266917 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
IHN47_RS15855 3266933..3268267 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
IHN47_RS15860 3268642..3270213 + 1572 WP_001273753.1 galactarate dehydratase -
IHN47_RS15865 3270362..3270697 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IHN47_RS15870 3270697..3271161 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IHN47_RS15875 3271216..3272025 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IHN47_RS15880 3272274..3273554 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
IHN47_RS15885 3273577..3274050 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
IHN47_RS15890 3274061..3274432 + 372 Protein_3103 PTS sugar transporter subunit IIC -
IHN47_RS15895 3274428..3274985 + 558 Protein_3104 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3261214..3282145 20931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T174050 WP_000347273.1 NZ_CP062248:3270697-3271161 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T174050 NZ_CP080521:97923-98315 [Pseudonocardia sp. DSM 110487]
GTGAAGGGTCTGCTGGCTACGAGCGTCTTCATCGCGTTGGAGAGCGGCAGGCCGCTCGACGAGCAGCGGATCCCCGATGA
GACAGCCGTGTCGGTGGTGACATTGGCGGAACTGCAGGCCGGTGTGCTGGCCGCGACGGACATCGACGTCCGAGCCCGTC
GCCTAGCCACGCTGGACGCTGTCGCTGATATCGAGTTGATCGACATTGATGAATCGGCCGCGCGTGCCTGGGCGCGGATG
CGTGTGCATCTGGCCGGGACTGGTCGACGGGTCAACGTCAACGATCTCTGGATCGCAGCGGTCGCCGCATCCCGCGGTCT
TCCGGTCGTGACGCAGGACGACGACTTCGGCCCGGTCGAGGGTGTCATGGGACTCGACGTCATCCGCGTCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT174050 WP_001307405.1 NZ_CP062248:3270362-3270697 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT174050 NZ_CP080521:97666-97926 [Pseudonocardia sp. DSM 110487]
ATGTCGAGCGTTCCCTCGCGTGAGCTCCGTAACGACACCGCGGGCGTTCTGCGACGAGTCGAGGCAGGTGAGCGCGTCAC
AGTCACGGTCAACGGCCGCCCGGTGGCCGAGCTGGTGCCGGTGCAGCGGTCCCGGAGGTCCTGGATCCGGCGCGACGAGC
TCGTCGCCCGGCTCCGTCGGGTTCAAGCGGATCCAGGGCTCCGTGACGACCTCGCCCGGCTCGCAGGCGACACGACTGAC
GACCTCGGACCCATCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References