Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 248492..249186 | Replicon | chromosome |
| Accession | NZ_CP062248 | ||
| Organism | Escherichia coli strain AML002_ev02 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q47157 |
| Locus tag | IHN47_RS01170 | Protein ID | WP_001263489.1 |
| Coordinates | 248788..249186 (+) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1QAE3 |
| Locus tag | IHN47_RS01165 | Protein ID | WP_000554758.1 |
| Coordinates | 248492..248785 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IHN47_RS01145 | 244124..244621 | + | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
| IHN47_RS01150 | 244845..246584 | - | 1740 | WP_001334802.1 | flagellar biosynthesis protein FlhA | - |
| IHN47_RS01155 | 246529..247314 | + | 786 | WP_000207552.1 | putative lateral flagellar export/assembly protein LafU | - |
| IHN47_RS01160 | 247385..248440 | + | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| IHN47_RS01165 | 248492..248785 | + | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| IHN47_RS01170 | 248788..249186 | + | 399 | WP_001263489.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| IHN47_RS01175 | 249196..249648 | + | 453 | WP_001059892.1 | GNAT family N-acetyltransferase | - |
| IHN47_RS01180 | 249966..250172 | + | 207 | Protein_228 | RtcB family protein | - |
| IHN47_RS01185 | 250168..250689 | + | 522 | Protein_229 | peptide chain release factor H | - |
| IHN47_RS01190 | 250746..252203 | - | 1458 | WP_001292994.1 | cytosol nonspecific dipeptidase | - |
| IHN47_RS01195 | 252464..252922 | + | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| - | 253518..253598 | + | 81 | NuclAT_12 | - | - |
| - | 253518..253598 | + | 81 | NuclAT_12 | - | - |
| - | 253518..253598 | + | 81 | NuclAT_12 | - | - |
| - | 253518..253598 | + | 81 | NuclAT_12 | - | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15487.84 Da Isoelectric Point: 7.4215
>T174018 WP_001263489.1 NZ_CP062248:248788-249186 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T174018 NZ_CP080494:3076306-3076887 [Pseudomonas sp. So3.2b]
ATGGACATCGAAAAAATCAAAGGGCGCCTCGAATTTCTACGCGAGGCGGAAAAGCTCAAGGACGTGCTTAGAAGCGCGCA
CACCTCTTGCGGGCGCACCGAAAGCACGGCGGAGCATAGCTGGCGCCTTTGCCTGATGGCCATTGTCTTTGGGGACTACC
TGACTGATCTTGATCGGCTGAAAATGCTCAAGATGTGTGTTATCCACGACTTGGGGGAGGCCATTAACGGAGATGTTCCC
GCCGTCAATCAAGATTCGTTTCCCAACAAGAGCGAGCAAGAGAGAAACGATCTCCTGCTCTTGACCAGTGCCCTGGATGA
ACCGTTGAAGGCCGAGATACTGGCGCTTTGGGATGACTATGAAAATGCTTCGTCGCCAGAGGCCGTAGCCGTCAAGGCGC
TGGATAAGTTGGAAACGATGCTGCAGCACAACCAAGGCAAGAACCCAACGGGCTTCGATTACGAATTCAATCTTGCCTAT
GGCAAAAAATACACAGATGCCGCGCCGTTGTTTGAGGTGCTGCGCACTATCATCGACCAGGAGACCAAAGAACACATACT
GGCGAATTCTAAAAATCACTAA
ATGGACATCGAAAAAATCAAAGGGCGCCTCGAATTTCTACGCGAGGCGGAAAAGCTCAAGGACGTGCTTAGAAGCGCGCA
CACCTCTTGCGGGCGCACCGAAAGCACGGCGGAGCATAGCTGGCGCCTTTGCCTGATGGCCATTGTCTTTGGGGACTACC
TGACTGATCTTGATCGGCTGAAAATGCTCAAGATGTGTGTTATCCACGACTTGGGGGAGGCCATTAACGGAGATGTTCCC
GCCGTCAATCAAGATTCGTTTCCCAACAAGAGCGAGCAAGAGAGAAACGATCTCCTGCTCTTGACCAGTGCCCTGGATGA
ACCGTTGAAGGCCGAGATACTGGCGCTTTGGGATGACTATGAAAATGCTTCGTCGCCAGAGGCCGTAGCCGTCAAGGCGC
TGGATAAGTTGGAAACGATGCTGCAGCACAACCAAGGCAAGAACCCAACGGGCTTCGATTACGAATTCAATCTTGCCTAT
GGCAAAAAATACACAGATGCCGCGCCGTTGTTTGAGGTGCTGCGCACTATCATCGACCAGGAGACCAAAGAACACATACT
GGCGAATTCTAAAAATCACTAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT174018 WP_000554758.1 NZ_CP062248:248492-248785 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT174018 NZ_CP080494:3075641-3076198 [Pseudomonas sp. So3.2b]
ATGTCTGACATGTTGTTAAGCCCTGTCTTACCCGGCGTTATGCTCAGGCGATGGCGCCTGTTACACCGGGTCAAACAATC
CCATGCCGCGGAGCTGTTTAACGTCACTCAATCGACTATCTCTCGCTGGGAGAGTGGCGTACTCGAGTTGGATCCTCTCG
CGCACAAAAAACTTGAGCAATTGCTGGCCGCTCGTCTCACCACCGCGGCGGATCAAGCGCTGGGACGAATGGTGCGGGAA
AGCTCGCGTCCTGTTCATCTCATGTGTGATTTGACGCACCGTCTGCTTGCTTGTTCTCCTTCGCGCGCCGCCCAGTTCAC
GAGTCCGGTCAGTGAACTGCTGGGTCGCTCGCTATGGCGCTTCGCTACGCCCAATATCATCAGCAACGAGCTCGCCCTGG
ATAAATTGGGCTGGCGGGAAGCCTTTGCGCCACCCGCGCTGGAGTTTCTGACGGGCCACAATAATTCGTCTATCGTTCCG
ATCAGCCAAAGCCTGTGTCGTTGGACGCGTATGCCTCTGTGCGACGGAACAACAGCGCGCTTGGTCGAGTCGCTCTGA
ATGTCTGACATGTTGTTAAGCCCTGTCTTACCCGGCGTTATGCTCAGGCGATGGCGCCTGTTACACCGGGTCAAACAATC
CCATGCCGCGGAGCTGTTTAACGTCACTCAATCGACTATCTCTCGCTGGGAGAGTGGCGTACTCGAGTTGGATCCTCTCG
CGCACAAAAAACTTGAGCAATTGCTGGCCGCTCGTCTCACCACCGCGGCGGATCAAGCGCTGGGACGAATGGTGCGGGAA
AGCTCGCGTCCTGTTCATCTCATGTGTGATTTGACGCACCGTCTGCTTGCTTGTTCTCCTTCGCGCGCCGCCCAGTTCAC
GAGTCCGGTCAGTGAACTGCTGGGTCGCTCGCTATGGCGCTTCGCTACGCCCAATATCATCAGCAACGAGCTCGCCCTGG
ATAAATTGGGCTGGCGGGAAGCCTTTGCGCCACCCGCGCTGGAGTTTCTGACGGGCCACAATAATTCGTCTATCGTTCCG
ATCAGCCAAAGCCTGTGTCGTTGGACGCGTATGCCTCTGTGCGACGGAACAACAGCGCGCTTGGTCGAGTCGCTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A090J8B1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1QAE3 |